IL-27基因多态性和血清IL-27p28水平与骨肉瘤风险相关性分析

Associations of IL-27 Polymorphisms and Serum IL-27p28 Levels with Osteosarcoma Risk

目的评估IL-27基因多态性和血清IL-27p28水平与骨肉瘤风险的相关性。方法对比研究160例骨肉瘤患者（研究组）和250例健康患者（对照组）。IL-27基因-964 A/G,2905 T/G,和4730 T/C多态性采用聚合酶链反应-限制性片段长度多态性测定。酶联免疫吸附试验检测血清中IL-27p28的水平。结果与对照组相比,骨肉瘤患者血清IL-27p28水平显著降低（P〈0.01）。Ⅲ-Ⅳ期骨肉瘤患者血清IL-27p28水平低于Ⅰ-Ⅱ期患者（P〈0.05）。同样,转移患者的血清IL-27p28水平低于非转移患者（P〈0.05）。IL-27-964 A/G,2905 T/G,4730 T/C的基因型和等位基因频率与骨肉瘤风险无相关性（P〉0.05）。分层分析也未能显示-964 A/G,2905 T/G,和4730 T/C多态性与临床分期和骨肉瘤的转移存在相关性（P〉0.05）。结论血清IL-27p28低水平可能与骨肉瘤进展有关,但IL-27基因-964 A/G,2905 T/G,和4730 T/C多态性和它们的单倍型不与骨肉瘤的风险相关联。

Objective To evaluate the correlation of L-27 gene polymorphism and serum levels of IL-27p28 and osteosarcoma risk. Methods A comparative study of 160 cases of osteosarcoma patients（ study group） and 250 healthy patients（ control group） were conducted. IL-27 gene-964 A / G,2905 T / G,and 4730 T / C polymorphism received polymerase chain reaction-restriction fragment length polymorphism determination. ELISA serum levels of IL-27p28. Results Compared with the control group,the serum levels of IL-27p28 of patients with osteosarcoma were significantly lower（ P〈 0. 01）. Serum levels of IL-27p28 of patients with osteosarcoma Ⅲ - Ⅳ were lower than below Ⅰ - Ⅱ patients（ P〈 0. 05）. Serum levels of IL-27p28 of patients with metastasis were lowere than non-metastasis patients（ P〈 0. 05）. IL-27-964 A / G,2905 T / G,4730 T / C genotype and allele frequencies and osteosarcoma risk had no correlation（ P〉 0. 05）. Stratified analysis failed to show the correlation of-964 A / G,2905 T / G,and 4730 T / C polymorphism with clinical stage and metastasis of osteosarcoma（ P〉 0. 05）. Conclusion Low serum levels of IL-27p28 maybe related with progression of osteosarcoma,but IL-27 gene-964 A / G,2905 T / G,and 4730 T / C polymorphism and their haplotypes are not associated risk of osteosarcoma.