基于微流控芯片的高糖对模式生物线虫寿命影响及白藜芦醇苷保护性作用考察

Microdevice for the investigation of high-glucose induced lifespan and the protective effect of polydatin in C. elegans

线虫是一种经典的模式生物,在分子和基因水平上与人类具有高度同源性,目前已被广泛应用于生命科学领域。本文以微流控芯片为平台,以模式生物秀丽隐杆线虫为研究对象,在单个线虫操作的水平上探讨高糖对线虫寿命和氧化应激的影响,同时考察了白藜芦醇苷对高糖引起的线虫寿命及应激响应变化的保护性作用。结果表明,高糖会缩短线虫寿命并增强线虫的氧化应激蛋白GST-4表达;而白藜芦醇苷会显著减弱高糖诱导的线虫应激反应并延长线虫寿命,表明白藜芦醇苷对高糖诱发的线虫应激反应具有保护性作用。本文所建立的单个线虫操控的微流控芯片可以为动物水平上治疗糖尿病的相关药物评价提供一种新的潜在平台。

Caenorhabditis elegans（C. elegans）has been widely used as a model organism for biomedical research due to its sufficient homology with human at molecular or genomic level. In this work,we describe a microfluidic device not only to investigate the response of C. elegans including lifespan and oxidative stress,but also to evaluate the protective effect of polydatin induced by high-glucose condition. It was found that the mean lifespan of worms was significantly reduced and the oxidative stress protein GST-4 was increased in worms that are subjected to high glucose. However,a certain dose of polydatin could weaken the increased oxidative stress induced by high-glucose and extend the lifespan,indicating the protective effect of polydatin against the toxic of high-glucose. The established approach is simple to operate,easy for realtime imaging and multiparatemer evaluations in parallel,providing a potential platform for drug evaluation / screening in a high throughput format at single animal resolution.