(4-环戊二烯基苯甲酸-铁-甲苯)六氟磷酸盐键合硅胶固定相的制备及色谱性能

Preparation and chromatographic performance of a silicabonded(4-cyclopentadienyl benzoic acid-iron-toluene)hexafluorophosphoric acid stationary phase

基于二茂铁独特的分子结构及其作为新型液相色谱分离基质的潜质,制备了新型(4-环戊二烯基苯甲酸-铁-甲苯)六氟磷酸盐键合硅胶固定相,利用红外光谱、元素分析、热重分析等手段对其进行了表征。分别选取多环芳烃、萘胺位置异构体、硝基苯胺位置异构体、硝基咪唑类化合物和有机磷化合物溶质作为探针,对其色谱性能进行了评价。结果表明,该色谱固定相能提供π-电子转移、π-π作用、偶极-偶极作用及与底物的静电相互作用等多种作用力,并探讨了可能的分离机理。

Based on the unique molecular structure of ferrocene and its potential as a new liquid chromatography separation medium,a new silica-bonded（4-cyclopentadienyl benzoic acidiron-toluene）hexafluorophosphoric acid stationary phase was prepared. The structure of this new material was characterized by infrared spectroscopy,elemental analysis,thermogravimetric analysis et al. The chromatographic performance and retention mechanism of this new stationary phase were evaluated using different solute probes,including polycyclic aromatic hydrocarbons（PAHs）,positional isomers of naphthylamine,positional isomers of nitro-aniline,nitroimidazoles,organic phosphorus et al. It could provide various action sites for different solutes in normal-phase chromatography such as π electron transfer,π-π electron interactions,dipole-dipole interactions,and electrostatic interactions with the substrates. And the possible separation mechanisms are discussed.