熊果酸对人卵巢癌SKOV3细胞增殖与凋亡的影响

Effect of Ursolic Acid on the Proliferation and Apoptosis of Human Ovarian Cancer SKOV3 cells

【目的】探讨熊果酸（Ursolic acid，UA）对人卵巢癌SKOV3细胞增殖与凋亡的影响。【方法】常规培养人卵巢癌SKOV3细胞，应用UA浓度为5、10、20、40和80μmol／L分别干预12h、24h和48h，采用MTT法检测细胞增殖；采用流式细胞仪检测细胞凋亡和细胞周期变化，应用Western blot技术检测细胞周期相关蛋白P21、Cyclin D1的表达水平。【结果】与空白对照组（0μmol／LUA）比较，40和80μmol／LUA分别作用SKOV3细胞12h、24h和48h后OD值均显著降低（P〈0．05）；10、20和40μmol／LUA作用24h后sKOV3细胞早期凋亡率、晚期凋亡率和G0／G1期比率较对照组升高，20和40μmol／LUA作用24h后SKOV3细胞周期蛋白Cyclin D1明显降低而P21水平显著升高（P〈0．05）。【结论】体外实验中，UA可抑制人卵巢癌SKOV3细胞增殖和促其凋亡，其机制可能与细胞周期蛋白Cyclin D1表达降低和P21表达升高有关。

[Objective]To observe the effect of ursolic acid （UA） on the proliferation and apoptosis of human ovarian cancer SKOV3 cells and explore its mechanism. [Methods]Human ovarian cancer SKOV3 cells were cultured conventionally and intervened using culture medium containing 5, 10, 20, 40 and 80μmol/L UA for 12h, 24h and 48h respectively. The proliferation of cells was observed by MTT assay; the apoptosis and cell cycle were detected using flow cytometry, and the expression levels of P21, CyclinD1 was detected by＇Western blot. [Results]Compared to the control group （UA 0 μmol/L ）, the OD of SKOV3 cells were significantly decreased. The early apoptosis rate, late apoptosis rate and G0/G1 phase ratio were higher than in the control group after using 10, 20 and 40μmol/L UA intervened for 24h （ P 〈0.05）. The protein level of Cyclin D1 significantly decreased while P21 significantly increased after 20 and 40μmol/L UA for 24h （ P 〈0.05）. [Conclusion]UA can significantly inhibit proliferation and promote apoptosis of human ovarian cancer SKOV3 cells in vitro, and its mechanism may be related to the decrease of cell cycle Cyclin D1 protein expression and the increase of P21 expression.