E2F3在浸润性膀胱癌中的表达关系以及对患者生存的影响

The clinical significance of E2F3 and RB expressions, and their effect on prognosis in bladder cancer

目的 探讨转录因子家族（E2F）3以及Retinoblastoma（RB）在膀胱癌组织中的表达情况,并明确RB通路失活与E2F3高表达在膀胱癌组织中的关系及对膀胱癌患者预后的影响.方法 通过免疫组织化学法验证E2F3和RB在112例膀胱癌组织中的表达,分析其表达水平与患者的临床分期、病理分级之间的关系.结果 在112例不同分期的膀胱癌组织样本中,有83例膀胱癌样本表现为RB的失活,这种表达差异具有统计学意义（P＜0.01）,E2F3高表达只出现在浸润性膀胱癌中（x2 =8.67,P＜0.01）,并且与RB失活关系密切（r=0.031,P=0.014）.生存曲线显示,E2F3过表达对膀胱癌患者预后有着一定的影响（P＜0.05）.结论 E2F3在浸润性膀胱癌中高表达,并且与RB失活存在着密切的关系,同时E2F3高表达的膀胱肿瘤患者预后更差.

Objective To investigate the expressions of transcription factor （E2F） 3 and retinoblastoma （RB）,and define the specific relationship between RB inactivation and E2F3 overexpression in individual bladder tumors.Methods The expression of E2F3 was investigate with immunohistochemistry in 112 bladder samples.The relationships between the expression of E2F3 and clinical grade,stage,and prognosis were analyzed.Results In 112 cases of tissue samples,83 cases of bladder cancer samples showed RB inactivation,this expression difference was statistically significant （P 〈0.01）;E2F3 appeared only in invasive bladder cancer （x2 =8.67,P 〈 0.01） with a close relationship with RB （r =0.031,P =0.014）.Survival curves were observed,overexpressed E2F3 has a worse prognosis （P 〈 0.05）.Concluo sions E2F3 was overexpressed in bladder cancer and was closely associated with RB inactivation.