兔动脉内膜剥脱模型的建立及血栓检测

Establishment of rabbit artery endarterectomy model and examination of thrombosis

目的:用金刚砂磨针建立兔动脉剥脱模型,模拟冠状动脉内膜剥脱术后血管腔内的病理过程。方法:分组将12只新西兰兔随机分成对照组和实验组,每组6只。实验组用金刚砂磨针磨除左颈动脉内膜建立内膜剥脱模型。取对侧颈动脉自身对照。术后2周取标本行HE染色及电镜检查。结果:用金刚磨针磨除内膜后,动脉损伤累及中膜,纤维蛋白原暴露,腔内表面不光滑;实验组血栓形成率高于对照组。结论:用金刚砂磨针建立动脉模型经济、可重复性强,可模拟CE术后血管腔内的病理过程。

Objective： To establish an artery endarterectomy model by using emery grinding needle and imitate the pathological changes of coronary artery after endarterectomy. Methods： 12 rabbits were randomly divided into two groups（ control group and experimental group） and each group had six rabbits. An emery grinding needle was used to establish the carotid artery endarterectomy model in the experimental group. The normal carotid artery was considered as own control in all rabbits. HE staining and electron microscopy examination were performed for every specimen. Results： Laceration of the tunica media,exposed fibrinogen and rough intravascular surface were observed after artery endarterectomy by using the emery grinding needle. Incidence of thrombosis was significant higher in the the experimental group compared with the control group（ P = 0. 015）.Conclusion： It＇s quite simple and stable to use emery grinding needle to establish an artery endarterectomy model,which can imitate the pathological changes of coronary artery after endarterectomy.