猴株五日热巴尔通体药敏及生化特性研究

Drug susceptibility and biochemistry characteristics of Bartonella quintana strains isolated from monkeys

目的了解猴株五日热巴尔通体的生化特性和药物敏感性,为进一步研究五日热巴尔通体的耐药机制奠定基础。方法采用E test法,检测1株五日热ATCC参考株及10株五日热巴尔通体猴分离株的生化特性及对14种抗生素的最低抑菌浓度(MIC)。结果 H15SC对利福平高度耐药(MIC>256),其余菌株敏感;H98SC对克林霉素耐药,其余菌株敏感;除S13外,其余10株菌株对丁胺卡那霉素、多粘菌素MICs值较高;全部菌株对阿奇霉素、头孢他啶、环丙沙星、庆大霉素、红霉素、妥布霉素、氯霉素、强力霉素、苄星青霉素敏感。结论首次发现巴尔通体菌株对利福平高度耐药,需要进一步了解其耐药机制,减少不合理用药,预防利福平耐药在人分离株中发生。

Objective To understand the biochemistry characteristics and drug susceptibility of Bartonella quintana strains isolated from monkeys and provide evidence to further study of drug resistance mechanism of Bartonella quintana. Methods E test was conducted to test the drug susceptibilities of 1 ATCC reference Bartonella quintana strain and 10 Bartonella quintana strains isolated from monkeys to 14 antibiotics. Results H15 SC strain was highly resistant to rifampicin（ MIC 256） and sensitive to other strain. H98 SC strain was resistant to clindamycin and sensitive to other strain. The other 10 stains were resistant to amikacin and polymyxin. All the stains were sensitive to azithromycin,ceftazidime,ciprofloxacin,gentamicin,erythromycin,tobramycin,chloromycetin,doxycycline,enzathine and benzylpenicillin. Conclusion It was the first time to found that Bartonella strain was highly resistant to rifampicin,but its resistant mechanisms needs further study. It is necessary to promote rational use of rifampicin to prevent the drug resistance of Bartonella strain.