可溶性有机质生物改性介导17β-雌二醇生物降解作用

Dissolved organic matters bio-modification mediated 17β-estradiol biodegradation

利用元素分析、紫外-可见光谱、三维荧光指数对经过15d生物改性前后腐殖酸的组分和结构进行表征,并比较了生物改性前后3种腐殖酸对17β-雌二醇(E2)的结合作用;而且研究了结合后的腐殖酸介导微生物降解E2的影响.结果表明:经过元素分析后,生物改性前的腐殖酸OLHA、OLFA、OSHA和生物改性后的腐殖酸BLHA、BLFA、BSHA的(N+O)/C值分别为0.801、1.214、0.820和0.629、1.080、0.797;紫外-可见光谱分析生物改性前后的SUVA_(254)指数分别为0.146、0.023、0.073和0.179、0.036、0.011;生物改性前后荧光指数(FI)分别为:0.723、3.385、2.757和0.681、3.017、1.702.上述3种表征手段分析得出腐殖酸的极性是一致的,即改性后的腐殖酸要比改性前的腐殖酸极性小.此外,在30h内5mgC/L的腐殖酸生物改性前后对3mg/L的E2的结合效率分别为31.37%、4.96%、25.86%和37.78%、6.03%、29.92%,明显发现改性后的腐殖酸对E2的结合作用增强;5 mgC/L的腐殖酸生物改性前后对3mg/L的E2的30h内的生物降解效率分别为46.28%、15.96%、38.76%和51.11%、17.30%、44.33%,而且同等浓度下的腐殖酸对E2的结合作用越大其介导微生物降解E2的效果越好.

Through elemental analysis, ultraviolet-visible spectra and fluorescence index, the composition and structure of the humic acid with and without 15 days biological modification were analyzed, and compared the binding function of three kinds of humic acid on 17β-estradiol（E2）. And the binding function between 17β-estradiol（E2） and three different kinds of humic acid that before and after bio-modification were then compared. At last, the microbial degradation influences of E2 mediated by humic acid were studied. The elemental analysis results showed that the（N＋O）/C values of humic acid（OLHA, OLFA, OSHA） that before bio-modification and humic acid（BLHA, BLFA, BSHA） that after biological modification were 0.801, 1.214, 0.820 and 0.629, 1.080, 0.797, respectively. The ultraviolet-visible spectrum results showed that the values of SUVA254 were 0.146, 0.023, 0.073 and 0.179, 0.036, respectively. And the fluorescence index（FI） were 0.723, 3.385, 2.757 and 0.681, 3.017, 1.702, respectively. The above three kinds of characterization analysis results showed that the polarity of humic acid were consistent, suggesting that the polarity of modified humic acid were weaker than former. Moreover, the binding efficiency of 3mg/L E2 by 5mg C/L three humic acid before and after the bio-modification within 30 h were 31.37%, 4.96%, 25.86% and 37.78%, 6.03%, 29.92%, respectively, which showed that the binding functions of E2 were increased obviously after humic acid bio-modification treatment. The biodegradation efficiency of 3mg/L E2 by 5mg C/L three humic acid before and after the bio-modification within 30 h were 46.28%, 15.96%, 38.76% and 51.11%, 17.30%, 44.33%, respectively. Meanwhile, the stronger binding function of the same humic acid concentrations on E2, the greater efficiency of combined humic acid mediated microbial degradation of E2.