正交设计法分析储存条件对艾叶挥发油成分的影响

Effects of storage conditions on volatile oil of Artemisia argyi leaves by orthogonal design method

【目的】利用正交设计法探讨不同储存条件对艾叶挥发油成分的影响,为其储存及开发利用提供技术参考。【方法】以艾叶为原料,在单因素试验基础上,分别以储存温度、储存湿度及储存时间为影响因素,以艾叶挥发油提取率及香气质量为评价指标,通过正交设计试验确定艾叶的最佳储存条件。【结果】影响艾叶储存效果的因素主次排序为:储存温度>储存湿度>储存时间,艾叶最佳储存条件为:储存温度40℃、储存湿度40%、储存时间8个月,挥发油平均提取率为0.645%;在此储存条件下得到的艾叶挥发油香气特征明显、丰富,经GC-MS分析,其主要成分有桉叶油醇(31.54%)、右旋龙脑(8.94%)、左旋樟脑(6.12%)和石竹烯(6.09%)等。【结论】采用正交设计法优化得到的艾叶储存条件能有效保持其挥发油成分含量和香气质量,可在实际生产中推广应用。

【Objective】The effects of different storage conditions on volatile oil of Artemisia argyi leaves were investigated by orthogonal design method, in order to provide references for storage, development and utilization of volatile oil of A. argyi leaves. 【Method】Using A. argyi leaves as raw materials, based on single factor experiment, the optimal storage conditions of A. argyi leaves were determined by orthogonal experiment, including storage temperature, storage humidity and storage time, with extraction rate and aroma quality as indexes. 【Result】The primary and secondary order of factors affecting storage of A. argyi leaves was as follows： temperature 〉humidity〉 time. And the optimal storage conditions for A.argyi leaves were as follows： storage temperature of 40 ℃, humidity of 40% and storage time of 8 months. Under the above optimum storage conditions, the average extraction rate of volatile oil was 0.645%, and the aromatic characteristics were more distinct and full-bodied. The GC-MS analysis showed that, the main components of volatile oil from A. argyi leaves were 1,8-cineole（31.54%）, L-camphor（8.94%）, D-camphor（6.12%） and Caryophyllene（6.09%） etc. 【Conclusion 】The storge conditions of A. argyi leaves optimized by orthogonal design method can maintain component content of volatile oil and aroma quality, which should be extended in production.