一株铁皮石斛内生真菌H1B1产红色素稳定性的研究

Study on stability of red pigments produced by an ednophytic fungus H1B1 from Dendrobium offi cinale

药用植物内生菌有产生活性物质的潜力,是天然功能色素的潜在来源之一。文章从温度、p H、光照、金属离子、氧化剂与还原剂、食品添加剂等方面探究铁皮石斛内生菌H1B1所产红色素的稳定性。结果表明,该菌所产红色素对温度、自然光较为稳定,对紫外线敏感;该色素在p H≥7环境条件下色素稳定性高且有增色效应;金属离子中,K^+、Mn^(2+)、Mg^(2+)、Co^(2+)、Al^(3+)、Cu^(2+)、Na^+、Zn^(2+)对该色素有护色或增色作用,但Fe^(3+)和Ca^(2+)对色素有明显的不良影响,容易产生沉淀;Na Cl和葡萄糖、蔗糖、麦芽糖等食品添加剂和配料能较好地保持红色素色价的稳定性,且10%~15%的葡萄糖对其有明显的增色效应。

Fungal pigments are one of the main sources of natural pigments. In this paper, we explored the effects of temperature, pH, light, metallic ions, oxidant and deoxidizer, food additives on the stabilities of the red pigment produced by the endophytes H1B1 from Dendrobium officinale. The results show that this red pigment was relatively stable to the temperature and light, but sensitive to UV. In the condition of pH ≥ 7, the pigment is stable and had effect on enhancing the red pigment. The red pigment was stable with K＋, Mn2＋. Mg2＋. Co2＋. Al3＋. Cu2＋, Na＋, Zn2＋, while Fe3＋ and Ca2＋ had obvious adverse effect which led to precipitation. Sodium chloride and glucose, sucrose, maltose could be used to maintain the stability of the color value, and 10%-15% glucose had significant color enhancement effect.