妊娠期父源抗原特异性激活调节性T细胞的研究

Study on role of paternal antigens-specific CD4^+CD25^+ T regulatory cells during pregnancy

目的探讨妊娠诱导调节性T细胞扩增与父源抗原的关系。方法建立正常妊娠(n=15)、流产倾向(n=15)、第三方交配(n=15)及假孕(n=6)共四组小鼠模型,观察调节性T细胞水平和激素含量变化。通过抗原特异性验证将父源抗原预免疫小鼠来源的调节性T细胞过继滴注到孕鼠体内,观察其对胚鼠的保护作用。结果流产倾向组与正常妊娠组孕鼠体内黄体酮、雌二醇、雌三醇水平比较,差异均无统计学意义(P>0.05)。滴注父源抗原特异性激活调节性T细胞或经父源抗原预免疫的调节性T细胞后,孕鼠流产率下降,调节性T细胞水平升高。结论妊娠期调节性T细胞扩增发挥保护作用的是父源抗原特异性激活,并非单一激素含量变化引起。

Objective To investigate that the pregnancy induced T regulatory（Treg） cells are generated by aiming at paternal antigens or induced by in vivo hormone change. Methods The 4 mice model groups were set up：normal pregnancy（n=15）,abortion trend（n=15）,third party mating（n=15） and false pregnancy（n=6）. The change of Treg cells level and hormone content were observed. The Treg cells originated from paternal antigen premunity mice was adoptively instilled into the pregnant mice by the antigens-specific verification for observing its protective effect on embryonic mouse. Results The in vivo progesterone,E2 and E3levels had no statistical differences between the abortion trend group and the normal pregnancy group（P〈0.05）. After instilling Treg cells of paternal antigen specific activation or adoptive transfer paternal antigens-specific Treg cells,the abortion rate of pregnant mice was decreased,while Treg cells level was increased. Conclusion The Treg cell amplification playing the protective role during pregnancy is paternal antigens specific activation,which is not caused by the single hormone content change.