PA-MSHA引起注射局部急性炎症及白细胞介素17、肿瘤坏死因子α和Toll样受体4的表达改变

Pseudomonas aeruginosa mannose sensitive hemagglutinin induced local acute inflammation in injected tissues

为研究皮下注射铜绿假单胞菌甘露糖敏感血凝菌毛株(Pseudomonas aeruginosa mannose sensitive hemagglutinin,PA-MSHA)注射液后局部组织的病理学改变及白细胞介素17(interleukin 17,IL-17)、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)和Toll样受体4(Toll-like receptor 4,TLR4)表达情况,将30只小鼠随机分为两组,分别皮下注射PA-MSHA和生理盐水,在注射后6、12、24、48及72h取注射局部组织,苏木精-伊红(hematoxylin-eosin,HE)染色后观察病理形态改变,并用免疫组化染色和图像分析检测IL-17、TNF-α和TLR4表达水平。结果显示,小鼠皮下注射PA-MSHA后,在给药部位引起以中性粒细胞浸润为主的急性炎症;局部炎症灶中IL-17、TNF-α和TLR4表达在注射后6h升高,24h达到高峰,然后逐渐减低,至72h实验组与对照组无差异;3种炎症因子的表达与炎症反应程度相平行。结果表明,皮下注射PA-MSHA能引起局部组织急性炎症反应,且能改变局部免疫状态。

To study the pathological changes and expressions of interleukin 17（IL-17）,tumor necrosis factorα（TNF-α）and Toll-like receptor 4（TLR4）in local tissues with administration of Pseudomonas aeruginosa mannose sensitive hemagglutinin（PA-MSHA）,30 mice were randomly divided into two groups,and received subcutaneous injection of PA-MSHA and normal saline respectively.The local injection tissues were sectioned at 6,12,24,48 and 72hafter injection,and the pathological changes and expressions of IL-17,TNF-αand TLR4 were detected by hematoxylin-eosin（HE）and immunohistochemical staining,and measured by image analysis. The results revealed that PA-MSHA caused acute inflammation with predominant neutrophil infiltration in the local injection tissues.In the inflammation lesions,the expression levels of IL-17,TNF-αand TLR4 were elevated at 6hafter injection,peaked at 24 h,then decreased gradually,and were comparable to the control group at 72 h.The expression levels of these three cytokines were consistent with the degree of inflammation.The data suggest that subcutaneous injection of PA-MSHA induces acute inflammation and changes the immunity state of local injection tissues.