摘要
文章建立了一种高效液相色谱方法对精油中常见的三种单萜化合物橙花醇、芳樟醇、香茅醇进行定性和定量分析,并进行了方法学验证。得到最佳色谱条件为:C18柱(4.6 mm i.d.×150 mm,5μm),以乙腈∶水=1∶1(v/v)为流动相,流速为1.0 m L/min,UV检测波长205 nm。同时验证了其精密度及方法的稳定性。结果表明,该方法灵敏度高,重复性好,方法学验证符合要求。进而采用此方法分析了玫瑰精油、柠檬精油、玫瑰香精、柠檬香精中三种单萜化合物的含量。
An analytical method based on high performance liquid chromatography was developed for analysis of nerol, linalool and citronellol, which are three main monoterpenes in essential oils. The optimized chromatographic conditions were determined as： C18 column （4.6 mm i.d. × 150 mm, 5 μm）, acetonitrile/ water （v/v, 1 ： 1） as mobile phase, UV detector （205 nm）, flow rate at 1.0 mL/min. The precision and stability of the method were also validated. This method is of high sensitivity, good reproducibility, and meets the methodological requirements. Furthermore, the methodology has been applied to detect the content of nerol, linalool and citronellol in rose oil, rose essence, lemon oil and lemon essence.
出处
《集成技术》
2016年第1期44-47,共4页
Journal of Integration Technology
基金
广东省自然科学基金项目(2015A030310317)
深圳市知识创新计划基础研究项目(JCYJ20150521144321007)
广州市科技计划项目产学研协同创新重大专项(201508020092)
关键词
精油
橙花醇
芳樟醇
香茅醇
高效液相色谱
essential oil
nerol
linalool
citronellol
high performance liquid chromatography
