HPLC法测定石黄清火丸中黄芩苷的含量

Determination of baicalin in Shihuang Qinghuo Wan by HPLC

目的：建立HPLC法测定石黄清火丸中黄芩苷的含量。方法：采用高效液相色谱法，以甲醇一水一冰醋酸（50：50：1）为流动相，检测波长为277nm。结果：黄芩苷进样量在1．0～2．0gg范围内呈良好的线性关系，r=0．9994，平均回收率为99．7％，RSD=1．82％（n=5）。结论：该方法简便、准确、专属性强，可用于测定石黄清火丸中黄芩苷的含量。

Objective： To survey content of baicalin in Shihuang Qinghuo Wan by HPLC. Methods： HPLC was applied. Mobile phase were methanol, water and ice vinegar （50： 50： 1）; And detection wavelength was 277nm. Results： Baicalin injection volume shows a good linear relationship during 1.0-2.0μg; r= 0.9994; the average recovery was 99.7%; RSD = 1.82% （n = 5）. Conclusion： This method is simple, convenient and accurate, and can be used to determine baicalin content of Shihuang Qinghuo Wan.