黄芪多糖对2型糖尿病大鼠血清IL-1β、IL-6、IL-18的影响作用

Effects of astragalus polysaccharides on serum IL-1β, IL-6 and IL-18 in diabetes Ⅱ rats

目的:观察黄芪多糖对糖尿病大鼠血糖的影响及血清IL-1β、IL-6、IL-18的影响作用,探讨黄芪多糖降糖的作用环节。方法:建立糖尿病大鼠模型,筛选出现多饮、多尿、多食,血糖升高(FBG≥16.7mmol/L)的对象作为糖尿病模型组,测量正常对照组及糖尿病模型组大鼠的血清FBG、FINS、IL-1β、IL-6、IL-18水平,然后将模型组随机分为四组,分别为黄芪多糖高剂量(0.8g·kg-1·d-1)、中剂量(0.4g·kg-1·d-1)、低剂量(0.2g·kg-1·d-1)及阳性对照(等量生理盐水灌胃)组。4周后空腹采集血液标本,尾静脉测大鼠血糖,放射免疫法测胰岛素,ELISA法测血清IL-1β、IL-6、IL-18。结果:1糖尿病模型组大鼠饮水、饮食量及尿量明显高于正常对照组,体重较前有明显下降,正常对照组无明显变化。2黄芪多糖治疗组饮水量、饮食量及体重减轻得到明显控制,阳性对照组无明显改善。3糖尿病模型组大鼠血清FBG、FINS、IL-1β、IL-6、IL-18水平明显升高,与正常对照组比较均有显著性差异(P<0.01),黄芪多糖治疗组较阳性对照组FBG、FINS、IL-1β、IL-6水平下降,差异有统计学意义(P<0.05);黄芪多糖治疗组IL-18水平低于阳性对照组,但无统计学意义(P>0.05);黄芪多糖高、中、低剂量组间FBG、FINS、IL-1β、IL-6、IL-18水平无显著差异(P>0.05)。结论:糖尿病大鼠血糖、血清胰岛素水平、IL-1β、IL-6、IL-18水平均升高,表明高脂+STZ诱导的糖尿病大鼠可出现胰岛素抵抗和IL-1β、IL-6、IL-18炎症因子的增高,IL-1β、IL-6、IL-18炎症因子可能参与了胰岛素抵抗的发生;黄芪多糖可降低糖尿病大鼠血糖水平及血清胰岛素水平,也可降低糖尿病大鼠血清IL-1β及IL-6,从而改善胰岛素抵抗,但对IL-18的影响作用不够显著。

Objective： To investigate efficacy of astragalus polysaccharides on serum IL-1β, IL-6, IL-18 in diabetic rats. Methods： To establish diabetic rat model, screening appears polydipsia, polyuria, polyphagia, hyperglycemia（FBG ≥ 16.7mmol/L） for the diabetic group, then the model group were randomly divided into four groups, namely high-dose Astragalus polysaccharide（0.8g·kg-1·d-1） group, middle dose（0.4g·kg-1·d-1）, low dose（0.2g·kg-1·d-1） and a positive control（saline gavage） group. Insulin radioimmunoassay, ELISA method were applied to measure serum IL-1β, IL-6, IL-18. Results： 1 Diabetic rats, drinking water, food intake and urine output were significantly higher than those in the control group; body weight decreased significantly compared with the previous. The control group showed no significant change. 2 Water intake, food intake and weight loss in treatment groups were significantly controlled. The control group showed no significant improvement. 3 FBG, FINS, IL-1β, IL-6, IL-18 levels were significantly increased, with significant difference（P〈0.01）. FBG, FINS, IL-1β, IL-6 levels in treatment groups were decreased; the difference was statistically significant（P〈0.05）; Levels of IL-18 in treatment groups were lower than the positive control group, but no statistical significance（P〉0.05）; FBG, FINS, IL-1β, IL-6, IL-18 among groups showed no significant difference（P〉0.05）. Conclusion： Glucose in diabetic rats, serum insulin levels, IL-1β, IL-6, IL-18 levels were elevated, suggesting that high-fat ＋ STZ-induced diabetic rats may be insulin resistance and IL-1β, IL-6, IL increased inflammatory factors-18, IL-1β, IL-6, IL-18 inflammatory factors may be involved in insulin resistance; Astragalus polysaccharides can lower blood sugar levels and insulin levels in diabetic rats, but also can reduce serum IL-1β and IL-6, thereby improving insulin resistance.