miRlet7b在氧糖剥夺诱导原代大鼠增殖星形胶质细胞的表达

The expression of miRlet7b in the primary proliferated astrocytes of oxygen-glucose deprivation rat

目的研究miRlet7b在氧糖剥夺诱导原代Wistar大鼠增殖星形胶质细胞的表达,探讨缺氧缺血性脑损伤后星形胶质细胞增殖的分子机制。方法原代Wistar大鼠星形胶质细胞随机分为实验组(氧糖剥夺诱导的增殖组)和对照组(正常培养组)。EdU掺入法检测两组细胞的增殖,Western blot检测VEGF蛋白表达,real-time PCR方法检测两组细胞miRlet7b mRNA的表达。结果实验组EdU阳性细胞率,VEGF蛋白表达水平显著高于对照组(P<0.01),而实验组miRlet7b mRNA的表达水平显著低于对照组(P<0.01)。结论 MiRlet7b mRNA表达下调可能是缺氧缺血性脑损伤后星形胶质细胞增殖的分子机制之一,上调VEGF可能促进星形胶质细胞增殖。

Objective To study the expression and possible mechanism of miRlet7 b in the primary proliferated astrocytes of oxygen-glucose deprivation（OGD） rat.Methods The primary rat astrocytes were divided randomly into control group（normal culture group） and experimental group（proliferation group induced by OGD culture）.The proliferation of primary rat astrocytes was measured by EdU incorporation,the expression of VEGF protein was detected by western blot,the expression of miRlet7 b was measured by real-time PCR.Results Edu positive ratio of primary astrocytes was significantly higher,the expression level of VEGF protein in the proliferated astrocytes was significantly increased in the experimental group than in control group（P〈0.01 or P〈0.05）,but the expression level of miRlel7 b in the proliferated astrocytes was lower in the experimental group than in control group（P〈0.01）.Conclusion The lower expression of miRlet7 b may be one of the molecular mechanisms in the proliferation of astrocytes,and upregulated VEGF possibly promotes the proliferation of astrocytes after hypoxic—ischemia brain injury.