摘要
采用传统的微生物培养和16S r DNA(V6-V8)高变区测序相结合的方法,从室内人工饲养的性成熟南亚实蝇雌虫肠道中分离、鉴定出21株细菌,均属于γ-变形菌门,分别为肠杆菌属(7株)、拉乌尔菌属(3株)、沙雷氏菌属(2株)、普罗维登斯菌属(2株)、不动杆菌属(1株)、柠檬酸杆菌属(1株)、西地西菌属(1株)和不可培养的细菌(4株);从性成熟的南亚实蝇雄虫肠道内分离得到20株细菌,分别为细菌(7株)、克雷伯氏菌属(4株)、肠杆菌属(3株)、普罗维登斯菌属(2株)、沙雷氏菌属(1株)、摩根菌属(1株)、假单胞菌属(1株)和γ-变形杆菌(1株),也均属于γ-变形菌门.由上可知,性成熟的南亚实蝇雌、雄成虫肠道共有细菌为肠杆菌属、普罗维登斯菌属和沙雷氏菌属,均属于肠杆菌科,表明肠杆菌科是南亚雌、雄成虫肠道细菌的优势菌群.
21 strains of bacteria were isolated and identified from the intestinal tract of sexually matured female Bactrocera tau which were reared in the laboratory using traditional isolation and 16 S r DNA( V6- V8 hypervariable region) sequencing technique. All bacteria strains belonged to the Gamma-proteobacteria phylum,which included Enterobacter( 7 strains),Raoultella( 3 strains),Serratia( 2 strains),Providencia( 2 strains),Acinetobacter( 1 strain),Citrobacter( 1 strain),Cedecea( 1 strain) and uncultured bacteria( 4 strains). Similarly,20 bacteria strains were isolated and identified from male B. tau and they also belonged to Gammaproteobacteria phylum,which consisted of bacteria( 7 strains),Klebsiella( 4 strains),Enterobacter( 3 strains),Providencia( 2strains),Serratia( 1 strain),Morganella( 1 strain),Pseudomonas( 1 strain) and gamma Proteobacterium( 1 strain). Bacteria genus which both females and males had in common were Enterobacter,Providencia and Serratia,which all belonged to Enterobacteriaceae. To summarize,Enterobacteriaceae was the dominant bacterium in intestinal tract of B. tau.
出处
《福建农林大学学报(自然科学版)》
CSCD
北大核心
2016年第1期8-13,共6页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家自然科学基金项目(11175046)
关键词
南亚实蝇
肠道
细菌
肠杆菌科
Bactrocera tau
intestinal tract
bacterium
Enterobacteriaceae