南亚实蝇成虫肠道细菌的分离与鉴定

Isolation and identification of bacteria in the intestinal tract of adult Bactrocera tau(Walker)(Diptera:Tephritidae)

采用传统的微生物培养和16S r DNA(V6-V8)高变区测序相结合的方法,从室内人工饲养的性成熟南亚实蝇雌虫肠道中分离、鉴定出21株细菌,均属于γ-变形菌门,分别为肠杆菌属(7株)、拉乌尔菌属(3株)、沙雷氏菌属(2株)、普罗维登斯菌属(2株)、不动杆菌属(1株)、柠檬酸杆菌属(1株)、西地西菌属(1株)和不可培养的细菌(4株);从性成熟的南亚实蝇雄虫肠道内分离得到20株细菌,分别为细菌(7株)、克雷伯氏菌属(4株)、肠杆菌属(3株)、普罗维登斯菌属(2株)、沙雷氏菌属(1株)、摩根菌属(1株)、假单胞菌属(1株)和γ-变形杆菌(1株),也均属于γ-变形菌门.由上可知,性成熟的南亚实蝇雌、雄成虫肠道共有细菌为肠杆菌属、普罗维登斯菌属和沙雷氏菌属,均属于肠杆菌科,表明肠杆菌科是南亚雌、雄成虫肠道细菌的优势菌群.

21 strains of bacteria were isolated and identified from the intestinal tract of sexually matured female Bactrocera tau which were reared in the laboratory using traditional isolation and 16 S r DNA( V6- V8 hypervariable region) sequencing technique. All bacteria strains belonged to the Gamma-proteobacteria phylum,which included Enterobacter( 7 strains),Raoultella( 3 strains),Serratia( 2 strains),Providencia( 2 strains),Acinetobacter( 1 strain),Citrobacter( 1 strain),Cedecea( 1 strain) and uncultured bacteria( 4 strains). Similarly,20 bacteria strains were isolated and identified from male B. tau and they also belonged to Gammaproteobacteria phylum,which consisted of bacteria( 7 strains),Klebsiella( 4 strains),Enterobacter( 3 strains),Providencia( 2strains),Serratia( 1 strain),Morganella( 1 strain),Pseudomonas( 1 strain) and gamma Proteobacterium( 1 strain). Bacteria genus which both females and males had in common were Enterobacter,Providencia and Serratia,which all belonged to Enterobacteriaceae. To summarize,Enterobacteriaceae was the dominant bacterium in intestinal tract of B. tau.