摘要
目的:建立同时测定连翘败毒丸中绿原酸、芍药苷、阿魏酸、盐酸小檗碱、黄芩苷含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent Eclipse XDB-C18,流动相为0.5%磷酸(加三乙胺调p H至3.0)-甲醇(梯度洗脱),流速为1.0 ml/min,柱温为30℃,检测波长分别为230 nm(芍药苷和盐酸小檗碱)、324 nm(绿原酸和阿魏酸)和278 nm(黄芩苷),进样量为5μl。结果:绿原酸、芍药苷、阿魏酸、盐酸小檗碱和黄芩苷检测质量浓度线性范围分别为1.884~30.144、0.392~6.272、0.102~1.632、1.326~21.216、1.95~31.2μg/ml(r=0.999 9、0.999 7、0.999 7、0.999 8、0.999 9);精密度、稳定性、重复性试验的RSD〈2%;加样回收率分别为99.58%~102.47%(RSD=0.93%,n=9)、99.21%~102.18%(RSD=0.90%,n=9)、98.28%~101.23%(RSD=0.86%,n=9)、99.66%~101.84%(RSD=0.82%,n=9)、99.18%~101.05%(RSD=0.62%,n=9)。结论:该方法准确可靠、操作简便快速,可用于连翘败毒丸中绿原酸、芍药苷、阿魏酸、盐酸小檗碱、黄芩苷含量的同时测定。
OBJECTIVE:To establish a method for the simultaneous determination of chlorogenic acid,paeoniflorin,ferulic acid,berberine hydrochloride and baicalin in Lianqiao baidu pill. METHODS:HPLC was performed on the column of Agilent Eclipse XDB-C18 with mobile phase of 0.5% phosphoric acid(triethylamine adjusted to p H 3.0)-methanol(gradient elution)at a flow rate of 1.0 ml/min,the column temperature was 30 ℃,the detection wavelength was 230 nm(for paeoniflorin and berberine hydrochloride),324 nm(for chlorogenic acid and ferulic acid)and 278 nm(for baicalin),and the injection volume was 5 μl. RESULTS:The linear range was 1.884-30.144 μg/ml for chlorogenic acid(r=0.999 9),0.392-6.272 μg/ml for paeoniflorin(r=0.999 7),0.102-1.632 μg/ml for ferulic acid(r=0.999 7),1.326-21.216 μg/ml for berberine hydrochloride(r=0.999 8)and 1.95-31.2 μg/ml for baicalin(r=0.999 9),respectively;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 99.58%-102.47%(RSD=0.93%,n=9),99.21%-102.18%(RSD=0.90%,n=9),98.28%-101.23%(RSD=0.86%,n=9),99.66%-101.84%(RSD=0.82%,n=9)and 99.18%-101.05%(RSD=0.62%,n=9). CONCLUSIONS:The method is accurate,reliable,simple and fast,and can be used for the simultaneous determination of chlorogenic acid,paeoniflorin,ferulic acid,berberine hydrochloride and baicalin Lianqiao baidu pill.
出处
《中国药房》
CAS
北大核心
2016年第6期815-818,共4页
China Pharmacy
基金
河北省自然科学基金资助项目(No.C2011105042)
关键词
高效液相色谱法
连翘败毒丸
绿原酸
盐酸小檗碱
黄芩苷
阿魏酸
芍药苷
含量测定
HPLC
Lianqiao baidu pill
Chlorogenic acid
Berberine hydrochloride
Baicalin
Ferulic acid
Paeoniflorin
Con tent determination