HPLC法同时测定连翘败毒丸中5种成分的含量

Simultaneous Determination of 5 Components in Lianqiao Baidu Pill by HPLC

目的：建立同时测定连翘败毒丸中绿原酸、芍药苷、阿魏酸、盐酸小檗碱、黄芩苷含量的方法。方法：采用高效液相色谱法。色谱柱为Agilent Eclipse XDB-C18,流动相为0.5%磷酸（加三乙胺调p H至3.0）-甲醇（梯度洗脱）,流速为1.0 ml/min,柱温为30℃,检测波长分别为230 nm（芍药苷和盐酸小檗碱）、324 nm（绿原酸和阿魏酸）和278 nm（黄芩苷）,进样量为5μl。结果：绿原酸、芍药苷、阿魏酸、盐酸小檗碱和黄芩苷检测质量浓度线性范围分别为1.884~30.144、0.392~6.272、0.102~1.632、1.326~21.216、1.95~31.2μg/ml（r=0.999 9、0.999 7、0.999 7、0.999 8、0.999 9）;精密度、稳定性、重复性试验的RSD〈2%;加样回收率分别为99.58%~102.47%（RSD=0.93%,n=9）、99.21%~102.18%（RSD=0.90%,n=9）、98.28%~101.23%（RSD=0.86%,n=9）、99.66%~101.84%（RSD=0.82%,n=9）、99.18%~101.05%（RSD=0.62%,n=9）。结论：该方法准确可靠、操作简便快速,可用于连翘败毒丸中绿原酸、芍药苷、阿魏酸、盐酸小檗碱、黄芩苷含量的同时测定。

OBJECTIVE：To establish a method for the simultaneous determination of chlorogenic acid,paeoniflorin,ferulic acid,berberine hydrochloride and baicalin in Lianqiao baidu pill. METHODS：HPLC was performed on the column of Agilent Eclipse XDB-C18 with mobile phase of 0.5% phosphoric acid（triethylamine adjusted to p H 3.0）-methanol（gradient elution）at a flow rate of 1.0 ml/min,the column temperature was 30 ℃,the detection wavelength was 230 nm（for paeoniflorin and berberine hydrochloride）,324 nm（for chlorogenic acid and ferulic acid）and 278 nm（for baicalin）,and the injection volume was 5 μl. RESULTS：The linear range was 1.884-30.144 μg/ml for chlorogenic acid（r=0.999 9）,0.392-6.272 μg/ml for paeoniflorin（r=0.999 7）,0.102-1.632 μg/ml for ferulic acid（r=0.999 7）,1.326-21.216 μg/ml for berberine hydrochloride（r=0.999 8）and 1.95-31.2 μg/ml for baicalin（r=0.999 9）,respectively;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 99.58%-102.47%（RSD=0.93%,n=9）,99.21%-102.18%（RSD=0.90%,n=9）,98.28%-101.23%（RSD=0.86%,n=9）,99.66%-101.84%（RSD=0.82%,n=9）and 99.18%-101.05%（RSD=0.62%,n=9）. CONCLUSIONS：The method is accurate,reliable,simple and fast,and can be used for the simultaneous determination of chlorogenic acid,paeoniflorin,ferulic acid,berberine hydrochloride and baicalin Lianqiao baidu pill.