秋子梨叶片高效再生体系的构建

Establishment of High Efficient Regeneration System of Pyrus ussuriensis Leaves

以秋子梨(Pyrus ussuriensis M.)叶片为外植体,NN69为基本培养基,研究了秋子梨组织培养高效再生体系。结果表明:噻隆(TDZ)对秋子梨叶片不定芽的诱导效果显著高于6-苄基腺嘌呤(6-BA)和激动素(KT),吲哚丁酸(IBA)和吲哚乙酸(IAA)的诱导效果显著高于萘乙酸(NAA)和2,4-二氯苯氧乙酸(2,4-D)。其中,适合秋子梨叶片不定芽再生的最佳培养基为NN69+TDZ 3.0mg/L+IBA 0.3mg/L,再生率和再生芽数分别为100.0%和8.47个;适合秋子梨不定根诱导的最佳培养基为1/2MS+IBA 0.5mg/L,生根率为45.5%,生根条数为2.24条。

In this study,an efficient regeneration system of leaves of Pyrus ussuriensis was established by using the NN69 basic culture medium. The results showed that introduction effect of TDZ was better than that of 6-BA and KT, introduction effect of IBA and IAA was better than NAA and 2,4-D, in adventitious bud regeneration. The optimal culture medium of shoot regeneration whose efficiency was 100. 0% and the number of shoots per plant was 8. 47, was NN69＋TDZ 3. 0 mg/L＋IBA 0. 3 mg/L. The optimal of root induction was 1/2MS＋IBA 0. 5 mg/L,and the efficiency of it was 45. 5% and root number was 2. 24 per shoot.