不同批次SD大鼠血清的代谢组学比较

Metabolomic Comparison of Serum Samples in Different Groups of SD Rats

基于核磁共振氢谱(1H NMR)的代谢组学及多元统计等技术分析不同饲养时期的健康SD大鼠血清,探讨了不同批次的实验动物在机体代谢上的差异性。结果表明不同批次的SD大鼠血清代谢物中,柠檬酸、丙酮酸、琥珀酸、谷氨酰胺、二甲基甘氨酸等成分的含量相对稳定,而丙氨酸、精氨酸、异亮氨酸、乳酸、3-羟基丁酸、胆碱等成分的含量易波动。进一步分析得出,氨基酸代谢、酮体代谢、胆碱代谢等体内代谢途径在不同批次大鼠间存在一定的波动性。该研究为评价健康大鼠之间的批次一致性提供了一种新的思路,也为准确地确定血液生物标志物奠定了基础。

To explore the differences in body metabolism of experimental animals,the serum samples of four batches of healthy SD rats raised in different periods were compared using proton nuclear magnetic resonance（1H NMR） based metabolomic approach combined with multivariate statistical analysis. The results showed that the contents of several serum metabolites in different groups,including citric acid,pyruvic acid,succinic acid,glutamine,dimethylglycine,changed slightly,while the amounts of alanine,arginine,isoleucine,lactic acid,3-hydroxybutyric acid and choline were fluctuant. Further metabolic pathways analysis showed that amino acid metabolism,ketone body metabolism and choline metabolism were fluctuant in different groups of SD rats. This study provided a new method for the evaluation on the consistency among the different groups of healthy SD rats,and laid a basis for further determination on the biomarkers in serum samples.