摘要
对雌核发育彭泽鲫(Carassius auratus var.Pengze,Pengze crucian carp,Pcc)中的Pcc-hira进行了克隆,序列分析,并对雌鱼9种组织和11个发育阶段中的表达进行了研究。结果显示,Pcc-hira CDS全长3028 bp,编码1009个氨基酸残基,具有7个典型WD40结构域,Pcc-HIRA与鲫HIRA具有99%的同源性,跟其他鲫属鱼具有较高的亲缘关系。Pcc-hira分别在孵化后36和44 d达到最高值和最低值,且在雌鱼脑中的含量极显著高于其他8种组织。
In the present study,Pengze crucian carp（ Pcc）-hira was cloned and characterized in the triploid gynogenetic fish,the Carassius auratus var. Pengze（ Pengze crucian carp）. Their mRNA expression profiling was investigated in eleven juvenile developmental stages and nine tissues of the adult female fish. Results showed that the complete CDS of Pcc-hira was 3028 bp encoding 1099 AA,containing seven classical WD40 domains. Multisequence alignment revealed that the deduced Pcc-HIRA protein shared the highest identities（ 99%） to Carassius auratus HIRA. The putative Pcc-HIRA shared high identities to HIRAs of crucian carp species. Gene expression profiling in the developmental stages showed that Pcc-hira had the highest / lowest expression at 36 /44 days post hatching（ dph）,respectively. Pcc-hira was predominantly expressed in brain tissue of the female fish compared with other 8 detected tissues.
出处
《淡水渔业》
CSCD
北大核心
2016年第1期18-23,28,共7页
Freshwater Fisheries
基金
National Natural Science Foundation of China(No.31270547)
