miR-26a对肝癌细胞侵袭迁移能力的影响及初步机制的研究

Effects and preliminary mechanism of miR- 26a on cell invasion and migration in the hepatocellular carcinoma cell line HepG2

目的:探讨miR-26a对人肝癌细胞株Hep G2侵袭迁移能力的影响及可能机制。方法:将miR-26a表达质粒及对照质粒分别转染Hep G2细胞,qRT-PCR检测转染后miR-26a的表达,细胞划痕实验和Transwell侵袭实验检测Hep G2细胞迁移和侵袭能力的改变,qRT-PCR和Western blot检测转染后AEG-1 mRNA和蛋白的表达。结果:转染miR-26a表达质粒后,Hep G2细胞miR-26a表达明显增高(P<0.01),迁移和侵袭能力显著降低(P<0.05),AEG-1 mRNA及蛋白表达水平明显下调(P<0.05)。结论:过表达miR-26a,可抑制Hep G2细胞迁移和侵袭,其机制可能与抑制AEG-1表达相关。

Objective： To explore the effect of miR- 26 a on the invasion and migration of hepatocellular carcinoma cell line Hep G2 and its mechanism. Methods： Hep G2 cells were transfected with miR- 26 a mimics or negative control,then transwell assay,wound healing assay were used for investigating cell invasion and migration,qRT- PCR and Western blot were performed to measure the expression of AEG- 1 mRNA and protein. Results： miR- 26 a was upregulated after transfected with miR- 26 a mimics in Hep G2 cells compared with NC,the ability of invasion and migration was significantly decreased（ P〈 0. 05）. Expression of AEG- 1 mRNA and protein was also downregulated observably（ P〈 0. 05）. Conclusion： Overexpression of miR- 26 a in Hep G2 cells may suppress cells invasion and migration,probably via downregulating its target gene AEG- 1 expression.