非小细胞肺癌血清miR-191的表达及意义

The expression of miR- 191 in serum of non- small cell lung cancer

目的:探讨血清miR-191对非小细胞肺癌(non-small cell lung cancer,NSCLC)的诊断价值及临床特征。方法:采用实时荧光定量PCR技术检测65例非小细胞肺癌患者血清和65例健康人血清中miR-191的表达量,分析血清miR-191的表达与非小细胞肺癌临床病理参数的关系,通过绘制ROC曲线分析其诊断非小细胞肺癌的价值。结果:非小细胞肺癌患者血清miR-191相对表达水平高于正常对照组(P<0.05),其表达与临床分期、病理类型及淋巴结转移显著相关(P<0.05),而与性别、年龄及吸烟史无相关性(P>0.05);miR-191的ROC曲线下面积AUC=0.951,其诊断非小细胞肺癌的灵敏度为87.3%,特异度为92.3%。结论:血清miR-191测定的诊断性能较高,有望作为诊断非小细胞肺癌的潜在分子标志物。

Objective： To study the expression of serum miR- 191 in patients with non- small cell lung cancer（ NSCLC） and its clinical significance. Methods： The level of serum miR- 191 was detected in patients with NSCLC,and the same number of normal subjects as control. Results： The expression level of serum miR- 191 was significantly higher in patients with NSCLC than that in the control（ P〈 0. 05）. The expression level of miR- 191 correlated with histology,TNM stages and Lymph node metastasis（ P〈 0. 05）,but didn＇t correlate with gender,age,smoking history（ P〈 0. 05）. The area under ROC curve was 0. 9 5 1,and the sensitivity and specificity of diagnosis were 87. 3% and92. 3% of miR- 191. Conclusion： Detection of miR- 191 in serum might be a potential mark of diagnosis and prognosis for NSCLC.