Ras和TβRII在外阴鳞状细胞癌中的表达及意义

Ras and TβRII expression in vulvar squamous cell carcinoma and its clinical significance

目的:探讨Ras和TβRII蛋白与外阴鳞癌发生发展的关系。方法:采用免疫组化法检测Ras和TβRII在外阴鳞癌中的表达情况,并结合临床病理资料进行分析。结果:Ras蛋白在外阴鳞癌的表达明显高于正常外阴皮肤,差异有统计学意义(P<0.05);在外阴鳞癌III期中表达明显高于I-II期(P<0.05);有淋巴结转移的表达明显高于无淋巴结转移(P<0.05);外阴鳞癌低、中分化和高分化之间表达没有统计学差异(P>0.05)。TβRII在外阴鳞癌的表达明显低于正常外阴皮肤(P<0.05);在外阴鳞癌III期中表达明显低于I-II期(P<0.05);中低分化的表达明显低于高分化(P<0.05);有淋巴结转移的表达明显低于无淋巴结转移(P<0.05)。Pearson相关性分析结果表明:在外阴鳞癌中Ras与TβRII蛋白的表达呈负相关。结论:Ras高表达和TβRII低表达可能与外阴鳞癌的发生、发展有关。

Objective： To investigate the expression of Ras and TβRII in vulva squamous cell carcinoma（ VSCC）,and its association with occurrence and developmen of disease. Methods： Immunohistochemistry was conducted to detect the expression of Ras and TβRII in both VSCC（ n = 25） and normal vulva tissues（ n = 15）,and analyzed the relationship between the expression of Ras,TβRII protein and clinical and pathological data of patients with VSCC. Results： The expression of Ras in VSCC was significantly higher than normal vulva skin tissue and the difference was statistically significant（ P〈 0. 05）. Ras expression in VSCC tissue of III stage was significantly higher than that of stage I- II（ P〈 0. 05）. The expression of Ras in cases with lymph node metastasis was higher than those without lymph node metastasis（ P〈 0. 05）. Ras expression had no statistically significant difference between the poorly and moderately differentiated VSCC and the highly differentiated ones（ P〉 0. 05）. The expression of TβRII in VSCC was significantly lower than normal vulva skin tissue（ P〈 0. 05）. TβRII expression in VSCC tissue of III stage was significantly lower than that of stage I- II（ P〈 0. 05）. The expression of TβRII in the moderately and poorly differentiated VSCC was lower than those well differentiated VSCC（ P〈 0. 05）. The expression of TβRII in cases with lymph node metastasis was lower than those without lymph node metastasis（ P〈 0. 05）. The Pearson correlation analysis showed that Ras and TβRII expression was negatively correlated in vulva squamous cell carcinoma. Conclusion： The expression of Ras and TβRII was associated with occurrence and development of VSCC.