AGXT2L1低表达对肝细胞癌患者预后的评价意义

Clinical Significance of the Down-Regulation of Alanine-Glyoxylate Aminotransferase 2-like 1 in Hepatocellular Carcinoma

目的:利用表达谱数据探讨AGXT2L1在肝细胞癌中的表达水平;分析AGXT2L1与肝细胞癌临床病理特征的关系;评价AGXT2L1对肝细胞癌预后评价的意义。方法:搜集NCBI的GEO公共数据集,对表达谱资料进行质检后,对样本及其对应的临床信息进行各临床相关指标的回顾性分析(卡方检验)和预后分析(Kaplan-Meier法);利用基因集富集分析(GSEA)的方法分析预测受AGXT2L1调控的相关基因。结果:在肝细胞癌中,AGXT2L1的表达显著低于癌旁组织;低表达AGXT2L1与患者甲胎蛋白(AFP)水平、肿瘤结节数目、肿瘤分期和转移潜能密切相关(P<0.05);低表达AGXT2L1的患者的总体生存期和无病生存期显著低于高表达组的患者(P<0.05);AGXT2L1可以影响与脂肪酸和脂质代谢相关的基因集。结论:AGXT2L1在肝细胞癌中显著低表达,与多个病理指标显著相关,其低表达与术后较差预后相关,并且AGXT2L1可以调控脂肪酸和脂质代谢。

Objective： To analyze the level of AGXT2L1 expression in hepatocellular carcinoma; to clarify the relationship between AGXT2L1 expression and elinicopathological characters of hepatoeellular carcino- ma; to evaluate the function of AGXT2L1 as a prognosis marker in hepatocellular carcinoma. Methods： GEO datasets were collected and tumor samples expression profile and clinical information were down- loaded. The correlation between gene expression level and clinicopathologic characters was analyzed by Chi-square test. Overall survival and recurrence-firee survival were analyzed by Kaplan-Meier curve. GSEA was used to predict the gene sets modulated by AGXT2L1. Results： The expression of AGXT2L1 was significantly lower in cancerous tissue compared with adjacent tissue （P〈0.05） ; AGXT2L1 expres- sion was associated with serum AFP level, tumor number, tumor stage and metastasis potential （P〈 0.05）; Lower expression of AGXT2L1 indicated poor prognosis in hepatocellular carcinoma; GSEA showed that AGXT2L1 affected gene sets about lipid and fatty acid metabolism. Conclusion： AGXT2L1 is down-regulated in tumor tissue and may function as a tumor suppressor in hepatocellular carcinoma via promoting excessive lipogenesis.