钛酸盐纳米管-聚苯胺-金纳米颗粒复合材料的制备及其生物电化学性能

Preparation of titanate nanotubes-polyaniline-gold nanoparticle composite and its bioelectrochemistry study

采用水热法制备出钛酸盐纳米管(TNTs),然后使用氧化聚合法将苯胺聚合到TNTs表面生成聚苯胺(PANI),最终将金纳米粒子还原到PANI表面制备出三元复合纳米材料(TNTs-PANI-GNPs).其中PANI作为交联剂链接起TNTs和GNPs形成稳定和结合紧密的三元复合纳米材料,不同的光谱和显微图像技术证实了三元复合纳米材料的成功合成.我们将辣根过氧化物酶(HRP)与三元复合纳米材料充分混合均匀后涂抹于玻碳电极表面,伏安实验结果证明HRP已实现在TNTs-PANI和TNTs-PANI-GNPs修饰电极上的直接电子转移,而且后者相较于前者对过氧化氢的催化还原电流增强了大约74.9%.TNTs-PANI-GNPs复合纳米材料修饰的生物传感器的优越电化学性能主要归因于复合材料中TNTs、PANI和GNPs三者之间的协同作用,将优点最大化,将缺点最小化.

A ternary composite consisting of titanate nanotubes（TNTs）,polyaniline（PANI）and gold nanoparticles（GNPs）was prepared for the first time and applied for the fabrication of a biosensor using horseradish peroxidase（HRP）as model enzyme.TNTs were initially synthesized from TiO2 nanoparticles using a hydrothermal method.Aniline was then polymerized on TNTs through an oxidation polymerization method.The GNPs were eventually deposited on PANI to form a ternary composite,in which PANI served as a cross linker between TNTs and GNPs to strengthen their contact.Different spectroscopic and microscopic techniques have confirmed the successful formation of the ternary composite.The ternary composite was then used for the immobilization of horseradish peroxidase（HRP）on electrode surface.The voltammetric results demonstrated that the direct electron transfer of HRP had been achieved at both TNTs-PANI and TNTs-PANI-GNPs modified electrodes and the catalytic reduction current at the later towards H2O2 has enhanced -74.9% compared to that at the former.The excellent electrochemical performance of the modified electrode can be ascribed to the superior synergistic properties of TNTs,PANI and GNPs.