果王素对小鼠肺腺癌GP方案化疗肝脏损害的防治作用

Effect of kiwi essence for prevention and treatment of liver injury caused by GP chemotherapy in mice with lung adenocarcinoma

目的本研究旨在观察果王素对肺腺癌小鼠使用GP方案化疗过程中对肝脏的保护作用,并初步探讨果王素护肝作用的相关机制。方法 32只接种Lewis肺腺癌的C57BL/6J小鼠按随机数字表法随机分成4组:对照组、化疗组、低剂量果王素联合化疗组及高剂量果王素联合化疗组。给予相应药物干预,于接种后24d处死各组小鼠,经眼球静脉丛取各组小鼠血液样本2mL,全自动生化分析仪检测肝功能相关指标,酶联免疫吸附法测定肿瘤坏死因子α(TNF-α)的浓度,测定肝匀浆中氧化及抗氧化酶活性,逆转录PCR(RT-PCR)检测肝脏TNF-αmRNA表达。结果化疗组有明显肝功能损害,予以果王素干预后,肝功能损害减轻;化疗组氧化应激指标升高,抗氧化酶活性降低,果王素干预后,氧化应激指标降低,抗氧化酶活性升高;化疗组血清中炎症因子TNF-α的浓度升高,肝脏中TNF-α表达增强,果王素干预后,TNF-α的表达降低。结论果王素能明显减轻化疗中肝功能损害的程度,起到护肝作用,其可能机制与清除氧自由基,抑制氧化应激及下调TNF-α表达抑制炎性反应有关。

Objective To investigate the liver protective effect of kiwi essence in the mice with lung adenocarcinoma during using GP chemotherapeutic regimen,and to explore its related mechanism.Methods Thirty-two C57BL/6Jmice bearing Lexis lung adenocarcinoma were randomly divided into 4groups：control group,chemotherapy group,low dose kiwi essence combined chemotherapy group and high dose kiwi essence combined chemotherapy group.The mice in each group were given the corresponding medication intervention and sacrificed on 24 d after inoculation for taking 2mL of blood sample by eye venous lexus.The automatic biochemical analyzer was used to detect the live function related indicators,the enzyme-linked immunosorbent assay（ELISA）was used to detect the tumor necrosis factor-α（TNF-α）level,the oxidase and antioxidase activities in liver homogenates were measured and the liver TNF-α mRNA expression was detected by using RT-PCR.Results The chemotherapy group had significant liver damage,which was alleviated after giving the kiwi essence intervention;the oxidation stress indicators in the chemotherapy group were elevated and the antioxidase activity was decreased,after kiwi essence intervention,the oxidation stress indicators were decreased,while antioxidase activity was increased;the serum inflammation factor TNF-αconcentration in the chemotherapy group was elevated,the TNF-αexpression in liver was enhanced,after kiwi essence intervention,the TNF-αexpression was decreased.Conclusion Kiwi essence could significantly reduce the degree of liver damage in chemotherapy and plays the liver-protecting effect.Its possible mechanism might be associated with scavenging the oxygen free radicals,inhibiting oxidative stress,down-regulating TNF-αexpression and inhibiting the inflammatory reactions.