新型坤净栓原味药中有效组分的测定

Content determination of effective component of natural medicine in new Kun Jing suppository

建立测定中药复方药新型坤净栓原味药柴胡和火绒草中各组分量的测定方法．采用高效液相色谱法．色谱柱为Hypersils C18（250nm×4．6nm），流动相为甲醇-水（梯度洗脱），流速为1．0mL／min，检测波长分别为：210nm和320nm，柱温为25℃．柴胡皂苷a：y=161．27x＋85．83，R2=0．9992；柴胡皂苷d：y=450．89x-254．78，R2=0．9991；咖啡酸：y=4333．9x-357880，R2=0．9997；其质量浓度分别在2．0～20．0μg，1．0—10．0μg线性范围内与各自峰面积积分值呈良好的线性关系；精密度、重复性、稳定性试验的RSD值分别为1．30％，1．22％，1．87％；平均加样回收率分别为98．30％，98．37％，97．65％，RSD值分别为2．39％，2．02％，3．42％（n=5）．该方法简便、灵敏、准确，可为新型坤净栓的质量控制提供依据．

To develop a method for content and edelweiss in Chinese herbal compound determination of natural medicine radix bupleuri new KunJing suppository. In this paper, the con- tent were analyzed using high - performance liquid chromatography （HPLC） analysis. Chro- matographic analysis was performed on a Hypersils ClsCOlumn （250 nm ×4.6 mm） ; the mobile phase was methanol and water with a constant flow rate of 1.0 ml/min. Peaks were detected at 210 nm and 320nm of UV detection. Column temperature was 25 ℃. Saikosaponin a： y = 161.27x ＋ 85.83, R2 = 0.999 2 ; Saikosaponin d ： y = 450. 89x - 254.78, R2 =0. 999 1 ; Caffeic acid ： y = 4 333.9x - 357 880, R2 = 0.999 7. The mass concentrations were in the range of 2.0 -20.0 μg and 1.0 - 10.0 μg, respectively, and have a good linear rela- tion with their peak area integral. The RSD values of precision, reproducibility, stability were 1.30% , 1.22% and 1.87%, respectively. The average sample recovery rates were 2. 39%, 2.02% and 3.42%, respectively. The established method was sensitive, simple, ac- curate applicable, and it provides the reference for the quality control of new KunJing sup-pository.