芝麻素对高糖条件下PC12细胞Aβ和BACE-1表达的影响

Effects of Sesamin on Aβ and BACE-1 Expression in PC12 Cells in High Glucose Culture Conditions

目的探讨芝麻素对高糖条件下鼠嗜铬细胞瘤细胞(PC12细胞)β位淀粉样前体蛋白裂解酶-1(BACE-1)和β淀粉样蛋白(Aβ)代谢的影响。方法培养PC12细胞,随机分为5组:对照组(正常培养组)、高糖培养组(25mmol/L)、高糖(25 mmol/L)加芝麻素0.5μmol/L组、高糖(25 mmol/L)加芝麻素5.0μmol/L组和高糖(25 mmol/L)加芝麻素50μmol/L组,酶联免疫吸附试验(ELISA)检测Aβ1-42水平,荧光实时定量PCR(RT-PCR)检测BACE-1 mRNA表达,Western blot检测BACE-1蛋白表达水平。结果高糖培养的PC12细胞组的Aβ1-42水平明显高于正常条件培养的PC12细胞组(P<0.05);在加入0.5、5.0和50μmol/L芝麻素后,Aβ水平显著降低(P<0.05);高糖培养的PC12细胞组BACE-1 mRNA和蛋白表达显著高于正常培养PC12细胞组(P<0.05);在加入0.5、5.0和50μmol/L芝麻素后,BACE-1 mRNA和蛋白水平显著降低(P<0.05)。结论在高糖条件下,PC12细胞Aβ1-42、BACE-1 mRNA和蛋白表达明显升高,芝麻素浓度依赖性地降低高糖环境下PC12细胞中升高的Aβ1-42水平,降低BACE-1 mRNA和蛋白表达。

Objective To explore the effects of Sesamin on the BACE-1 and Aβ expression in Rat Pheochromocytoma cells （PC12 ceils） in high glucose culture conditions. Methods The PC12 cells were randomly divided into five groups： Control group （PC12 cells in normal culture conditions）, PC12 cells in high glucose culture conditions （25 mmol/L）, PC12 cells in high glucose conditions （25 mmol/L） plus Sesamin 0. 5 μmol/L,5.0 μmol/L and 50 μmol/L. Aβ1-42 concentration was measured by ELISA. BACE-1 mRNA and protein expression was measured by RT-PCR and Western blot. Results Compared with PC12 cells under normal conditions, the Aβ1-42 level, the BACE-1 mRNA and protein expression were elevated in PCI2 cells in high glucose conditions （P 〈 0. 05 ） ;meanwhile, the AIM-42 concentration, BACE-1 mRNA and protein ex- pression in high glucose was significantly decreased after intervened by 0. 5,5.0 and 50 I.Lmol/L Sesamin （ P 〈 0. 05 ）. Conclusion The Aβ1-42, BACE-1 mRNA and protein expression are increased in PC12 cells in high glucose conditions, Sesamin concentration-dependently reverse the increasing Aβ1-42 ,BACE-1 mRNA and protein level in PC12 cells in high glucose conditions.