基于硝酸消解法的蛋白琥珀酸铁口服溶液含量测定

Determination of Iron in Iron Proteinsuccinylate Oral Solution with Nitric Acid Digestion

目的:优化现有蛋白琥珀酸铁口服溶液含量检测方法。方法:采用硝酸消解法替代现有盐酸消解法预处理样品,以完全水解多肽链并释放结合铁,采用2,2'-联吡啶络合物比色法测定铁含量,以琥珀酸酰化酪蛋白为空白载体考察回收率,以市售样品考察重复性。结果:硝酸消解法和盐酸消解法的回收率分别为99.62%和90.36%,市售样品含量测定RSD分别为0.44%和3.50%。结论:硝酸消解法替代盐酸消解法可显著改善蛋白琥珀酸铁口服溶液含量检测方法的可靠性。

Objective：To establish a reliable determination method for iron in iron proteinsuccinylate oral solution. Methods：samples were pretreated with nitric acid digestion instead of the existing hydrochloric acid digestion to fully hydrolyze polypeptide chain and release the complexing iron,the iron content was determined using 2,2 ＇- pyridine complex colorimetry,the recovery and repeatability were evaluated using proteinsuccinylate and commercially available sample respectively. Results：with nitric acid digestion or hydrochloric acid digestion,the recovery was 99.62% or 90.36% respectively,RSD of the iron content 0.44% and 3.50% respectively. Conclusion ：nitric acid digestion instead of hydrochloric acid digestion can significantly improve the reliability of determination method of iron in the iron proteinsuccinylate.