高效液相法测定颠茄磺苄啶片中硫酸阿托品含量

Determination of Atropine in Belladonna Sulfamethoxazole and Trimerhoprim Tablets by HPLC

目的：建立测定颠茄磺苄啶片中硫酸阿托品含量的高效液相色谱（HPLC）法。方法：以十八烷基硅烷键合硅胶为填充剂,0.8%三乙胺溶液（用磷酸调节p H值至2.9）-乙腈（85∶15）为流动相,检测波长为210nm,柱温为室温。结果：硫酸阿托品在12.56~200.96μg/m L范围内线性关系良好;r=0.9998;平均回收率为96.79%（n=6）,RSD为1.4%。结论：该方法简便、快速、准确并且专属性强。

Objective：To establish a HPLC method for determination of Atropine in Belladonna Sulfamethoxazole and Trimerhoprim Tablets. Methods：The HPLC System consisted of Gemini Phenomenex C18 column,0.8% triethylamine solution（adjust p H value to2.9 with phosphoric acid）- acetonitrile（85 ∶15） as mobile phase,with detection wavelength 210 nm. Results：The linear range of atropine was 12.56~200.96μg/m L. The mean recovery was 96.79%（n=6）.conclusion：The method is proved to be simple,rapid,accurate and exclusive.