地黄饮子脑脊液药物化学研究

Identification and Analysis of Constituents Absorbed into Cerebrospinal Fluid after Oral Administration of Di Huang Yin Zi

目的:阐明经方地黄饮子在脑脊液中的化学成分分布情况。方法:利用UPLC-ESI-MS和GC-MS技术分析地黄饮子的体外样品及灌胃给予地黄饮子复方或单味药后的家兔脑脊液样品,对比分析各样品结果,确认灌胃给予地黄饮子后的家兔脑脊液中移行成分、代谢产物及其生药来源。结果:灌胃给予地黄饮子后家兔脑脊液中发现5个移行成分,其中1个为地黄饮子所含的原型成分,4个为新产生的代谢产物。结论:对地黄饮子的脑脊液中移行成分及代谢产物进行了确认,对其进行深入研究,有助于阐明地黄饮子治疗中枢退行性病变的有效成分及作用机理。

Objective： To identification and analyze the constituents absorbed into cerebrospinal fluid of Di Huang Yin Zi. Method： Two valid methods using Ultra Performance Liquid Chromatography coupled with electrospray ionization（ESI） Mass spectrometry and Gas chromatography – Mass spectrometry were established for the study of the absorbed components in rabbit cerebrospinal fluid after oral administration of Di Huang Yin Zi. Result： Five compounds including one original form compound and four metabolites were observed from a comprehensive analysis of the chromatography of Di Huang Yin Zi, controlled cerebrospinal fluid and dosed cerebrospinal fluid.Conclusion： This study developed a method for screening the bioactive constituents in cerebrospinal fluid after oral administration of Chinese herbal medicine and provided helpful chemical information for further pharmacology and active mechanism research on TCM.