鹿茸血酒对去势大鼠骨密度与T细胞亚群的影响

Effects of Pilose Antler Blood Wine on Bone Mineral Density and T-lymphocyte Subsets in Ovariectomized Female Rats

目的探讨鹿茸血酒对于去势Sprague Dawley(SD)雌性大鼠股骨骨密度和T细胞亚群的影响。方法 60只SD雌性大鼠按随机数字表法分为6组:正常对照组、骨质疏松模型组、单纯乙醇干预组、高剂量鹿茸血酒组、中剂量鹿茸血酒组、低剂量鹿茸血酒组。正常对照组、骨质疏模型组给予生理盐水9.1 g/(kg·d),单纯乙醇干预组给予单纯乙醇干预9.1 g/(kg·d),高、中、低剂量鹿茸血酒组剂量分别为:13.6、9.1、4.5 g/(kg·d)。造模后第4天开始,干预30 d,干预第31天各组大鼠断头处死,取右侧股骨进行骨密度测定,取颈动脉全血运用流式细胞术测定大鼠T细胞亚群。结果高、中、低剂量鹿茸血酒组与骨质疏松模型组比较,均可明显提高去势大鼠的骨密度(P<0.05),其中以中、低剂量提高骨密度的作用更为明显(P<0.05)。同时,鹿茸血酒组与骨质疏松模型组比较,可提高去势大鼠CD4+细胞百分比及CD4+/CD8+比值,降低去势大鼠CD8+细胞百分比(P<0.05)。中剂量组提高去势大鼠的CD4+细胞百分比与高剂量组相比明显(P<0.05)。低、中剂量组提高去势大鼠的CD4+/CD8+比值的效果比高剂量组明显(P<0.05)。中剂量鹿茸血酒与单纯乙醇干预组比较,CD4+百分比升高(P<0.05)。低、中剂量鹿茸血酒与单纯乙醇干预组比较,CD8+百分比降低(P<0.05)。低、中、高剂量鹿茸血酒与单纯乙醇干预组比较,CD4+/CD8+比值升高(P<0.05)。结论 3种剂量鹿茸血酒均可减少大鼠骨量丢失,防止骨密度降低,预防骨质疏松症,对免疫功能有改善作用,其中中、低剂量组对骨质疏松和免疫功能改善明显。

Objective To explore the effects of pilose antler blood wine on bone mineral density and T-lymphocyte subsets of ovariectomized female Sprague Dawley（SD） rats. Methods Sixty female SD rats were randomly divided into six groups： normal control group, osteoporosis model group, pure ethylalcohol group, high dose of pilose antler blood wine group, medium dose of pilose antler blood wine group and low dose of pilose antler blood wine group. Normal control group and osteoporosis model group were given saline with the dose of 9.1 g/（kg·d）. Pure ethylalcohol group was given pure ethylalcohol with the dose of 9.1 g/（kg·d）. The doses of pilose antler blood wine were 4.5, 9.1 and 13.6 g/（kg·d） respectively in low, medium and high dose of pilose antler blood wine group. After the feeding of pilose antler blood wine for 30 days, the bone mineral density and T-lymphocyte subsets of rats were assessed. Results The difference in bone mineral density among the three pilose antler blood wine groups was significant（P〈0.05）; the most obvious improvement of one mineral density was found in low and medium dose of pilose antler blood wine groups（P〈0.05）. The CD4＋ T cells and CD4＋/CD8＋ significantly increased and CD8＋ T cells proportion decreased significantly in pilose antler blood wine group compared with those in the osteoporosis model group（P〈0.05）. CD4＋ T cells in medium dose group increased obviously compared with that in the high dose group（P〈0.05）. CD4＋/CD8＋ in low and medium dose groups obviously increased compared with that in high dose group（P〈0.05）. CD4＋ T cells of medium dose group obviously increased compared with that in pure ethylalcohol group（P〈0.05）. CD8＋ T cells in medium and low dose groups obviously decreased compared that in pure ethylalcohol group（P〈0.05）. CD4＋/CD8＋ in the three dose groups increased significantly compared with that in pure ethylalcohol group（P〈0.05）. Conclusions Tree different doses of pilose antler blood wine could prevent the osteoporosis and improve the immune function. The effects of medium and low dose of groups were more obvious.