光敏化姜黄素的细胞毒理学检验

Cytotoxicological Test of Photoactivated Curcumin

探究基于小鼠成纤维L929细胞的光敏化姜黄素的细胞毒理学。采用倒置显微镜观察细胞形态;四甲基偶氮唑蓝(methyl thiazolyl tetrazolium,MTT)法检测L929细胞的相对增殖率,按通用标准评价细胞毒性;DNA片段化分析及Hoechst细胞核染色法检测细胞凋亡状况。结果表明:经不同浓度的光敏化姜黄素(5、10、20μmol/L)处理后,L929细胞生长良好,呈梭形或不规则形状,与对照组相比均无明显差异;毒性级别为"0"或"1",即无细胞毒性;在琼脂糖凝胶电泳图上未见"阶梯状"的条带出现,细胞核染色后也呈现正常的蓝色,即光敏化姜黄素不会引起L929细胞凋亡,对L929细胞没有毒性。

The main purpose of this research was to explore the cytotoxicity of photoactivated curcumin based on fi broblast cell line L929. The cell morphology was examined by an inverted microscope, relative growth rate（RGR, %） was detected by methyl thiazolyl tetrazolium（MTT） method, cytotoxicity was evaluated according to the general standard, and cell apoptosis was detected by DNA ladder analysis and Hoechst nuclear staining, respectively. Results showed that photoactivated curcumin did not affect cell morphology, which was fusiform or irregular in shape and had no signifi cant difference when compared with the control group. It had no cytotoxicity with the toxicity grade ＂0＂ or ＂1＂, and did not cause apoptosis according to DNA ladder analysis and Hoechst nuclear staining. This study confi rmed that photoactivated curcumin had no toxicity to L929 cells at the concentrations of 5, 10 and 20 μmol/L. These results will lay a foundation for exploring the safety of consumption of photoactivated curcumin and provide the theory basis for the popularization and application of curcumin-mediated photodynamic non-thermal sterilization technology in daily life.