国内部分地区多杀性巴氏杆菌荚膜血清型和基因型的研究

Identification of capsule serotype and genotype of Pasteurella multocida in some areas of China

为了解国内部分地区不同动物多杀性巴氏杆菌(P.multicida)流行情况,本研究以36株P.multicida为研究对象,通过多重荚膜PCR、多位点序列分型(MLST)和脂多糖多重PCR(LPS-m PCR)对其荚膜型和基因型进行鉴定。采用多重荚膜PCR方法将36株细菌的荚膜血清型分为A、B和D 3种,其中禽源P.multicida主要以A为主;以LPS-m PCR方法将P.multicida分为L1、L2、L3和L6 4种LPS基因型,禽源P.multicida主要为L1型;通过MLST技术将P.multicida分为ST-129、ST-8、ST-58、ST-5、ST-13、ST-50和ST-122 7种ST型,禽源P.multicida主要为ST-129型。本研究为P.multicida的流行病学监测和基因多样性提供了技术支撑。

To investigate the epidemic status of the Pasteurella multocida isolates from different hosts in some areas of China,thirty-six P.multocida isolates were isolated from the clinical samples collected from 6 provinces,and subject to identifications of the capsule types and genotypes by multiplex capsular PCR,MLST and LPS-m PCR.The isolates were found to be members of capsule serotypes A,B,and D based on capsular serotyping using multiplex capsular PCR,of which the avian original isolates were mainly classified into capsule type A.Four different LPS genotypes,included L1,L2,L3,and L6,were identified by LPS-m PCR,and avian original isolates were mainly in L1.Seven different sequence types（STs） including ST-129,ST-8,ST-58,ST-5,ST-13,ST-50,and ST-122,were identified by MLST,and avian original isolates were mainly for ST-129.These results demonstrated MLST and LPS-m PCR approach are useful for epidemiological surveillance and determination of the genetic diversity of P.multocida prevalance.