一株N,N-二甲基甲酰胺高效降解菌的筛选鉴定及其生物降解特性

Screening and Identification of High-Effective Degradation Strains and the Biodegradation Characteristics for DMF Removal

从山东某制药厂污水处理系统的活性污泥中驯化、筛选分离得到1株能够以N,N-二甲基甲酰胺(DMF)作为唯一碳源、氮源和能源进行生长的细菌,命名为DM-2。根据菌落形态观察、生理生化特征和16S r DNA基因序列同源性对比分析对其进行鉴定,发现该菌为需氧、革兰氏阴性菌,氧化酶呈阳性反应,吲哚试验呈阴性反应,不分解山梨糖,且该菌的16S r DNA序列与副球菌属(Paracoccus huijuniae)的相似度达99%,因此,初步鉴定该菌为副球菌属。通过摇瓶试验考察了温度、p H和接种量对菌株DM-2降解DMF性能的影响,试验结果表明该菌生长和降解DMF的最适培养条件为温度30℃,p H7.0,最适接种量为5%。在最适培养条件下,DM-2在72 h内对初始浓度为5 000 mg/L DMF的去除率达到95%。研究结果对高效降解DMF生产废水具有重要的应用价值。

A bacteria strain DM -2 with high efficiency of DMF-degrading was isolated from a wastewater treatment pool containing DMF in a pharmaceutical plant. This strain utilised DMF as the sole source of carbon and nitrogen, was identified as Paracoccus huijuniae based on 16S rRNA gene sequence analysis, morphological and physiological properties. The optimal temperature, pH and inoculation for the growth of DM -2 were 30℃, 7.0 and 5% , respectively. DM -2 was able to degrade 95% of 5 000 mg/L DMF within 72 hours under the best biodegradation conditions. The research has an important application value for efficient degradation of DMF production wastewater.