中国新记录种毛细木霉Trichoderma capillare及功能评价

Chinese new record Trichoderma capillare and its functional assessment

木霉菌株TW22166分离自湖南省洞庭湖滩涂湿地,该菌株分生孢子梗具有长而发达的主轴,二次分枝丰富,不规则,一般单生,分生孢子梗向顶方向着生单个瓶梗。利用ITS、tef1及rpb2序列分析,发现该菌株同Trichoderma capillare同源性达到99%以上;在以rpb2构建的系统发育进化树中,TW22166与Trichoderma capillare strain GJS 06-66[JN175530.1]位于同一分支。该菌株在PDA平板上对9种植物病原真菌均有抑制作用,其中对Fusarium.oxysporum的抑制率为100.0%。菌株具有蛋白酶、几丁质酶及纤维素酶活性,其中蛋白酶活性较高,透明圈宽度达16.0 mm。该菌株鉴定为毛细木霉Trichoderma capillare,是木霉属中国新记录种,也是一株较有潜力的生物防治菌株。

Trichoderma strain TW22166 was isolated from soil samples of Dongting Lake mudflat wetland of Hunan province, China. Its conidiophores had a strongly developed central axis from which phialides arise singly over several levels below the tip a single phialide terminating a basal cell with a short, spur-like phialide arising as an outgrowth of the basal cell at the septum. Through detecting molecular markers （ITS, tefl and rpb2）, we discovered that its homology with Trichoderma capillare was more than 99% by NCBI. In rpb2 sequences based phylogenetic tree, strain TW22166 and Trichoderma capillare strain GJS 06-66 E JN175530.11 were on one identical branch. Strain TW22166 exhibited antifungal capability against 9 plant pathogenic fungi on PDA plate, and its inhibition ratio against Fusarium oxysporum was up to 100.0%. It had proteinase, chitinase and cellulase activity, among which proteinase activity was higher and its hydrolyzation radius was 16.0 mm. Strain TW22166 was identified as Trichoderma capillare, a Chinese new record of genus Trichoderma which was a potential biological prevention and control strain.