干旱胁迫下甘蓝型油菜相关抗旱基因的表达分析

Expression analysis of Brassica napus L.drought-related genes under drought stress

以抗旱性较强的甘蓝型油菜Holiday为材料,在开花初期对油菜进行干旱胁迫处理,采用RT-qPCR技术分析ABA2、BnSOS2、BnCS、CAM、CBF4、PIP1这6个油菜抗旱相关基因在干旱胁迫第1天、3天、5天、7天在根、茎、叶、花和青荚中的表达量.结果表明,干旱胁迫下,6个抗旱相关基因在油菜的不同器官中均出现了上调表达;在不同干旱胁迫下,各基因表达量呈现不同的变化趋势;在相同的器官中,各基因的表达量存在明显的不同,累积表达量表现为根中ABA2最大、CBF4最小,茎中CAM最大、CBF4最小,叶中PIP1最大、ABA2最小,花中CBF4最大、BnSOS2最小,青荚中BnCS最大,CBF4最小.说明植物在受到干旱胁迫时,不同的抗旱途径对干旱胁迫的响应程度是不同的;不同器官中各抗旱相关基因与胁迫时间的相关性分析表明,CAM基因在茎中的表达量、CBF4基因在花中的表达量与胁迫时间呈显著正相关.

The high drought-tolerant variety Holiday was treated as the experimental material under drought stress in the early flower stage.The expression level of 6 drought-related genes（ABA2、BnSOS2、BnCS、 CAM、CBF4、PIP1） which were selected from different organs（root,stem,leaf,flower and green pod） at different stress times（1,3,5,7 days） were analyzed by qRT-PCR technology.The result showed that the expression of six drought-resistant genes in different organs all increased under drought stress,and they presented different trends respectively under different drought stress.In the same organs,the expression of the six drought-resistant genes had obvious different.In root,the maximum cumulative expression value gene was ABA2,and the minimum cumulative expression value gene was CBF4;in stem,the maximum was CAM,the minimum was CBF4;in leaf,the maximum was PIP1,the minimum was ABA2;in flower,the maximum was CBF4,the minimum was BnSOS2;in green pod,the maximum was BnCS,the minimum was CBF4.It suggested that different drought resistance pathway played different role under drought stress.The correlation analysis between expression values of drought-related genes and stress time showed that the expression quantity of CAM gene in the stem volume,CBF4 gene in the flowers was significantly positively related to the stress time.