HPLC法同时测定甘草总黄酮分散片中甘草素和甘草查尔酮A的含量

Simultaneous Determination of Liquiritigenin and Licochalcone A in Licorice Flavonoids Dispersible Tablets by HPLC

目的建立同时测定甘草总黄酮分散片中甘草素和甘草查尔酮A含量的方法。方法采用高效液相色谱法,Inertsil C18柱（4.6 mm×150 mm,5μm）,流动相以乙腈（A）-0.1%磷酸水（B）梯度洗脱（0-8 min,25%-32%A;8-30 min,32%-45%A;30-42 min,45%-60%A;42-50 min,60%-90%A）,柱温30℃,检测波长为276 nm,365 nm,流速1.0 mL/min。结果甘草素和甘草查尔酮A的线性范围分别为0.016 1-0.644 0μg（r=0.999 8）,0.019 3-0.771 2μg（r=0.9997）。甘草素平均回收率为99.16%,RSD=1.51%;甘草查尔酮A平均回收率为98.48%,RSD=1.22%。结论该法简便、灵敏度高、重现性好,可作为甘草总黄酮分散片中指标性成分的含量测定和成品质量控制。

OBJECTIVE To establish a method for simultaneous determination of liquiritigenin and licochalcone A in Licorice flavonoids dispersible tablets by HPLC. METHODS Inertsil C18column（ 4. 6 mm × 150 mm,5 μm） was used with mobile phase of Acetonitrile-0.1% H3PO4 soiution gradient elution（ 0-8 min,25%-32% A; 8-30 min,32%-45% A; 30-42 min,45%-60% A; 42-50 min,60%-90% A）. The detection wavelength was set at 276 nm,365 nm. The flow rate was 1.0 mL / min and the column temperature was maintained at 30 ℃. RESULTS The linear relationship of liquiritigenin and licochalcone A were 0.016 1-0.644 0 μg（ r = 0.999 8）,0.019 3-0.771 2 μg（ r = 0.999 7）. The average recovery rate were 99. 16% with RSD 1. 51%; 98. 48% with RSD 1. 22%（ n = 6）,respectively.CONCLUSION The established method is simple,accurate and reliable. It can be used for determination of the 2components and quality control in Licorice flavonoids dispersible tablets.