甲基丙二酸血症患者血清miRNAs表达及其意义分析

Plasma circulation microRNAs expression profile in patients with methylmalonic acidemia

目的初步探讨循环血清微小RNA（miRNAs）在甲基丙二酸血症（MMA）患者中的表达规律，寻找以miRNAs为代表的MMA新的生物标记物和治疗评价指标。方法选取自2011年8月至2013年6月就诊于郑州大学第一附属医院及郑州大学第三附属医院的10例临床上晚发型维生素B12有效型MMA患者作为实验组，选取患者未患病的健康亲属8例作为对照组。尿样本经气相色谱-质谱（GC／MS）定量测定甲基丙二酸倍率。常规留取血清，抽提miRNAs；实时定量PCR法评价样本质量，微阵列基因芯片检测各组循环血清miRNAs的表达水平；应用R软件芯片预测分析方法（PAM）对芯片数据进行预测性分析，筛选出差异表达的miRNAs。结果GC／MS结果显示，实验组尿甲基丙二酸倍率与对照组相比明显上升；实验组治疗后尿甲基丙二酸倍率与治疗前相比明显下降。实时定量PCR结果显示血清样本中提取的RNA符合实验要求，可进入下游芯片实验。统计学分析结果显示，实验组与对照组相比、实验组治疗前后相比均有多个miRNAs表达差异有统计学意义（P〈0．05）。R软件PAM结果显示mir-483、mir-144表达在实验组较对照组明显上调，差异有统计学意义（P〈0．05）；mir-151、mir-30、mir-146表达在实验组较对照组明显下调，差异有统计学意义（P〈0．05）。结论MMA患者循环血清中存在多个miRNAs表达异常，这些有可能成为MMA的生物标记物。

Objective To analyze the differential expressions of microRNAs in patients with methylmalonic acidemia （MMA） and explore the expression rule ofmicroRNAs in MMA pathogenesis primarily to find the new biomarkers and therapeutic evaluation index of MMA represented by microRNAs. Methods Ten patients, admitted to our hospitals and diagnosed as having delayed onset vitamin B 12 valid MMA fi＇om August 2011 to June 2013, were chosen as experimental group （MMA group）; and their 8 healthy relatives were chosen as negative control group （NC group）. Plasma microRNAs were routinely extracted and filed, and samples quality was evaluated with real-time quantitative PCR; microarray gene chip was employed to detect the expression levels ofmicroRNAs; R software of prediction analysis of microarrays （PAM） was used to filter differentially expressed miRNAs. Results The results of GC/MS showed that the urine methylmalonic acid zoom ratio of MMA group was significantly higher than that of NC group. And the urine methylmalonic acid zoom ratio of MMA group had remarkable difference between before and after treatment. Real-time PCR showed the RNAs extracted from plasma samples conformed to the requirement of experiment and could be delivered to downstream chip experiment. The chip statistical analysis suggested that there were many micrornas enjoying significant differences between MMA group and NC group （P〈0.05）, and differences in MMA group before and after treatment （P〈0.05）. The resluts of R software of PAM indicated that mir-483 andmir-144 were strongly raised in MMA group as compared with those in NC group （P〈0.05）, while mir- 151, mir-30 and mir-146 were remarkably reduced in MMA group as compared with those in NC group （P〈0.05）. Conclusion There are several abnormal expressions of plasma circulation microRNAs in patients with methylmalonic acidemia; the plasma circulation microRNAs might be biomarkers of methylmalonic acidemia.