IL-10在细粒棘球蚴感染小鼠的免疫调控作用研究

The expression of IL-10 in mice infected with Echinococcus

目的通过检测细粒棘球蚴感染小鼠IL-10表达水平的变化,探讨IL-10的免疫调控作用。方法收集感染细粒棘球蚴病羊肝脏囊泡中的原头蚴,处理后接种实验组小鼠肝脏,建立实验动物模型。于感染后2、8、30、90和270d各取8只小鼠处死,收集血液,分离血清,采用ELISA检测IL-10含量;同时剥取小鼠脾脏,采用RT-PCR检测IL-10mRNA。实验设0.9%NaCl对照组。结果与对照组比较,实验组小鼠在感染后60和90d血清IL-10表达水平显著升高,在感染后180d达到峰值,随后略有下降,但持续高水平表达(t180d=-27.087,t270d=-19.382,均P<0.01)。与对照组相比,实验组小鼠脾脏IL-10mRNA水平在感染后90d达到峰值(t=-3.959,P<0.01),之后表达量仍然处于较高水平状态(t180d=-2.571,t270d=-3.284,均P<0.01)。结论细粒棘球蚴感染后期小鼠IL-10持续高表达,这可能是导致细粒棘球蚴发生免疫逃逸的机制之一。

Objective To determine the immunoregulatory effect of IL-10 by detecting the level of IL-10 in mice infected with Echinococcus granulosus. Methods Mice were randomly divided into two groups,an experimental group（agroup infected with E.granulosus）and a control group（saline group）.Eight mice were randomly selected from the experimental group and the control group 2,8,30,90,180,and 270 days after infection.Blood was collected and the spleen was removed and tested for IL-10 mRNA with ELISA and RT-PCR. Results The serum levels of IL-10 were lower in the control group and the serum levels of IL-10 in the experimental group changed significantly 60 and 90days after infection.The serum concentration of IL-10 was 63.03±5.24,and this concentration peaked 180 days after infection（t180d=-27.087,P180d=0.000;t270d=-19.382,P270d=0.000）.In the experimental group,the level of expression of IL-10 mRNA in the spleen was 1.83±0.46.This level peaked 90 days after infection and remained high 180 and 270days after infection（t180d=-2.571,P180d=0.022;t270d=-3.284,P270d=0.005）. Conclusion RT-PCR and ELISA were used to track IL-10 in mice at different times during infection with E.granulosus.The data suggest that IL-10 increases in the late stage and remains at a high level,which probably how E.granulosus evades the immune system.