青海高原牦牛PRDM16基因克隆、生物信息学及组织差异表达分析

Cloning,Bioinformatics and Tissue Differential Expression Analysis of PRDM16 Gene in Qinghai Plateau Yak

本研究对青海高原牦牛PRDM16(PR domain containing 16)基因部分编码区进行克隆及生物信息学分析,同时对PRDM16基因在雌、雄牦牛背最长肌肌肉组织中的表达差异进行了分析。选取雌、雄青海高原牦牛各5头,屠宰后采集背最长肌肌肉组织,克隆牦牛PRDM16基因部分CDS区序列,分析其生物信息学特征;应用实时荧光定量PCR技术检测PRDM16基因在雌、雄牦牛肌肉组织中表达水平。结果显示:克隆所得片段序列长323bp,与黄牛同源性为100%,编码99个氨基酸,具有MDS1-EVI1(complex locus protein MDS1)家族蛋白功能,具有CATH蛋白功能活性,包含卷曲螺旋等典型结构域,与人甲基转移酶蛋白结构域PR蛋白1有20%的相似性;PRDM16基因在雌性牦牛肌肉组织中表达水平极显著高于雄性牦牛(P<0.01)。本试验结果为进步一研究青海高原牦牛PRDM16基因奠定了基础,为牦牛肉品质分析提供参考。

In this study,the part coding region of PRDM16 gene of the Qinghai plateau yak was cloned and its bioinformatics and differential expression of PRDM16 gene of muscle tissue were analyzed in different sex individual.The PRDM16 gene part of CDS region was cloned from the longissimus dorsi muscle tissue of Qinghai plateau yak,analyzed its characteristics of bioinformatics and detected gene expression levels of different sex individuals in muscle tissue by Real-time PCR.The results showed that the sequence length of the cloned fragment was 323 bp,the homology between Qinghai plateau yak and cattle was 100%.It encoded 99 amino acids,containing domains of MDS1-EVI1（complex locus protein MDS1）family proteins function,CATH protein function and typical structure of the curly spiral.PRDM16 protein was 20% of the similarity with people methyltransferase protein domain PR protein 1.PRDM16 gene expression of female yak was significantly higher than male yak＇s in longissimus dorsi muscle tissue（P〈0.01）.The test results provided not only research foundation for genetic progress but also technical references foryak meat quality analysis.