不同断奶日龄对仔猪脾脏生长相关基因表达的影响

Effect of Different Weaning Age on Related-growth Gene Expressions of Spleen in Piglets

为研究不同断奶日龄对仔猪脾脏中生长激素受体(growth hormone receptor,GHR)、胰岛素样生长因子-1(insulin like growth factor-1,IGF-1)和胰岛素样生长因子-1受体(insulin like growth factor-1receptor,IGF-1R)基因表达的影响,试验选取体重相近、健康的杜×长×大仔猪24头,随机分为4组(14、21、28和35日龄断奶组),每个试验组6个重复,每个重复1头仔猪,至42日龄时全部宰杀取样,用RT-PCR方法检测脾脏中上述基因的相对表达水平。结果表明,14和21日龄断奶组仔猪脾脏GHR mRNA的相对表达量显著高于35日龄断奶组(P<0.05);不同断奶日龄组仔猪脾脏中的IGF-1mRNA的相对表达量均无显著性变化(P>0.05);35日龄断奶组仔猪脾脏中IGF-1R mRNA的相对表达量显著高于28日龄断奶组(P<0.05)。结果提示,不同断奶日龄对仔猪脾脏相关生长基因表达影响的模式不同;不同断奶日龄脾脏IGF-1R mRNA的表达可能受脾脏自身局部IGF-1的调控。

In order to study the effect of different weaning age on related-growth gene expressions of spleen in piglets,24 similar body weight and healthy piglets were selected（Duroc×Landrace×Yorkshine）.All piglets were randomly divided into 4groups（14,21,28 and 35days of age weaning groups）,and each group had 6replications including 1piglet separately.They were slaughtered at 24 days of age and spleen samples were acquired.The relative expression of growth hormone receptor（GHR）,insulin like growth factor-1（IGF-1）,insulin like growth factor-1receptor（IGF-1R）mRNA in spleen were measured by RT-PCR.The results showed that the relative expression of GHR mRNA in 14 and 21days of age weaning groups was significantly higher than that in 35 days of age weaning group（P〈0.05）;The relative expression of IGF-1 mRNA in spleen at different weaning age groups was no significant difference（P〈0.05）;The relative expression of IGF-1R mRNA in 35 days of age weaning group was significantly higher than that in28 days of age weaning group（P〈0.05）.The results indicated that the patterns of weaning age on related growth gene expressions of spleen are different;The expressions of spleen IGF-1R mRNA in different weaning age groups were possibly effected by the spleen self regulation of IGF-1.