原花青素对玉米赤霉烯酮致小鼠肝脏、肾脏氧化损伤的保护作用

Protective Effects of Proanthocyanidins on the Liver and Kidney Oxidative Damage Induced by Zearalenone in Mice

试验旨在研究原花青素（PC）是否对玉米赤霉烯酮（ZEA）中毒的小鼠肝脏及肾脏具有保护作用。选取40只日龄为8-9周、体重约为45g的健康清洁级雄性昆明系小鼠,随机分为4组,对照组灌喂生理盐水,PC组灌喂100mg/kg PC,ZEA组灌喂40mg/kg ZEA,PC＋ZEA组灌喂100mg/kg PC＋40mg/kg ZEA,眼球采血,颈椎处死后采取肝脏样本。测定肝脏组织中谷丙转氨酶（ALT）、谷草转氨酶（AST）、丙二醛（MDA）、超氧化物歧化酶（SOD）含量及血清中尿酸（UA）、尿素氮（BUN）含量。结果显示,与对照组相比,PC组各项指标差异均不显著（P〉0.05）,提示肝脏及肾脏没有明显的氧化损伤;与对照组相比,ZEA组的小鼠组织中谷草转氨酶、谷丙转氨酶及丙二醛含量显著或极显著升高（P〈0.05;P〈0.01）,超氧化物歧化酶含量极显著降低（P〈0.01）,血清中尿酸、尿素氮含量极显著升高（P〈0.01）,提示肝脏及肾脏有严重的氧化损伤;PC＋ZEA组的小鼠各项指标（除超氧化物歧化酶外）均显著或极显著低于ZEA组（P〈0.05;P〈0.01）,提示其肝脏及肾脏氧化损伤程度低于ZEA组,PC对ZEA中毒小鼠的肝脏及肾脏氧化损伤有一定的保护作用。

The experiment was conducted to study whether the proanthocyanidin （PC） had the protective effects on the liver and kidney oxidative damage induced by zearalenone in mice. 40 8 to 9 week-old healthy clean grade Kunming mice with the 45 g body weight were chosen and ran- domly divided into four groups： control group was given normal saline, PC group was given 100 mg/kg PC,ZEA group was given 40 mg/kg ZEA and PC＋ZEA group was fed 100 mg/kg PC＋40 mg/kg ZEA. Liver and blood sampls were taken after cervical vertebra to death. AST. ALT, MDA.SOD were measured to determine the degree of liver damage and antioxidant capacity, and UA and BUN were used to determine the kidney damage. The results showed that： Compared with the control group,the indexes of PC group were not significant changed （P〈0.05）, indicating that there was no obvious oxidative damage in the liver and kidney. Compared with control group,ALT, MDA and AST content were significantly or extremely significant increased in ZEA group （P〈0.05 ;P〈0.01） ,SOD content was extremely significant decreased （P〈0.01） ,UA andBUN were extremely significant increased in serum （P〈0. 01） ,indicating that the liver and kid- ney of ZEA group had a serious Oxidative damage. The indexes of the PCq-ZEA group （except SOD） were significantly or extremely significant lower than ZEA group （P〈0.05 ;P〈0.01） ,indi- cating that the oxidative damage of the liver and kidney in PCq-ZEA group was lower than that in the ZEA group. In conclusion,the liver and kidney oxidative damage of mice induced by ZEA was eased to some extent by PC.