莱菔硫烷对镉中毒雄性小鼠生殖机能保护作用的研究

Protective Effect of Sulforaphane on Reproductive Function of Male Mice with Cadmium Poisoning

本试验旨在研究莱菔硫烷(SFN)对染镉雄性小鼠生殖机能的保护作用。选择健康的清洁级雄性昆明系小鼠40只,随机分成4组,分别为对照组(H2O)、镉组(2.3mg/kg CdCl2)、莱菔硫烷组(10mg/kg SFN)、镉+莱菔硫烷组(10mg/kg SFN+2.3mg/kg CdCl2),连续给药10d,于最后一次给药2d后脱颈处死,检测小鼠睾丸组织病理学变化、小鼠睾丸及附睾的脏器系数、精液品质及血清睾酮浓度。同时检测睾丸组织内丙二醛(MDA)、谷胱甘肽(GSH)含量及总超氧化物歧化酶(T-SOD)活性的变化。结果表明,与对照组相比,镉组小鼠的睾丸组织发生病理学损伤,睾丸及附睾的脏器系数、精液品质及血清睾酮浓度均极显著降低(P<0.01),睾丸组织T-SOD活性、GSH含量也极显著降低(P<0.01),MDA含量极显著提高(P<0.01);莱菔硫烷组T-SOD活性和GSH含量显著升高(P<0.05),MDA含量与对照组相比差异不显著(P>0.05)。与镉组相比,镉+莱菔硫烷组小鼠的精子活率、精子总数极显著增加(P<0.01),睾丸与附睾的脏器系数显著增加(P<0.05),小鼠睾丸组织GSH含量、T-SOD活性极显著提高(P<0.01),MDA含量极显著降低(P<0.01)。以上结果指出莱菔硫烷对镉中毒雄性小鼠的生殖机能具有保护作用。

The study was aimed to explore the protective effect of sulforaphane（SFN）on the reproductive function of male mice with cadmium poisoning.40 healthy clean grade male Kunming mice were randomly divided into four groups：control group（H2O）,cadmium chloride group（2.3mg/kg CdCl2）,sulforaphane group（10 mg/kg SFN）,sulforaphane ＋ cadmium chloride group（10mg/kg SFN＋2.3mg/kg CdCl2）,and continuous administration for 10 d,all mice were executed by dislocated cervical vertebra at 2dafter the last administration,and then the pathologic changes of testicular tissues,organ coefficient of testicle and epididymis,sperm quality and concentration of testosterone were tested.Additionally,the contents of GSH and MDA,and the activities of T-SOD in testis were also detected at the same time.Compared with the control group,pathology damages were observed in cadmium chloride group,organ coefficient of testis and epididymis,sperm quality and levels of testosterone extremely significantly decreased（P〈0.01）,the activities of T-SOD and GSH content were extremely significantly decreased（P〈0.01）,and the concentration of MDA was extremely significantly enhanced（P〈0.01）.Compared with the con-trol group,the activity of T-SOD and concentration of GSH in sulforaphane group were significantly increased（P〈0.05）,and the concentration of MDA was not significant different between the control group and sulforaphane group（P〈0.05）.While compared with the cadmium chloride group,the sperm motility rate and sperm total count in sulforaphane and cadmium chloride group were extremely significantly increased（P〈0.01）,the organ coefficient of testicle and epididymis was increased significantly（P〈0.05）,the concentration of GSH and activity of T-SOD in testicular tissue were extremely significantly increased（P〈0.01）,and the concentration of MDA was extremely significantly decreased（P〈0.01）.The results indicated that sulforaphane had the protection effect on reproduction function of male mice with cadmium poisoning.