期刊文献+

RH-HPLC法测定青蒿素中脱氢青蒿素及9-表-青蒿素的含量 被引量:3

Determination of artemisitene and 9-epi-artemisinin in artemisinin raw material by RH-HPLC
下载PDF
导出
摘要 建立了一种利用反相液相色谱法测定青蒿素原料药中脱氢青蒿素和9-表-青蒿素含量的分析方法。采用紫外检测器于210 nm处进行检测,以C-18柱(Agilent Zobax ODS,150 mm×4.6 mm,5μm)为分析柱,以乙腈和水的混合溶液为流动相,采取梯度洗脱方式(0~10 min,45%乙腈;10~20 min,45%~80%乙腈;20~25 min,80%~45%乙腈;25~35 min,45%乙腈)进行洗脱。在此条件下,青蒿素、脱氢青蒿素和9-表-青蒿素能彼此完全分离。脱氢青蒿素和9-表-青蒿素的浓度分别在4.11~28.76、9.42~65.93μg/m L范围内与峰面积的线性关系良好,相关系数R均大于0.998,检出限(3S/N)分别为2.668 ng和75.34 ng,样品加标回收率均在94%~108%之间。 A reversed-phase high performance liquid chromatography (RP-HPLC) is developed for the determination of artemisitene and 9-epi-artemisinin in artemisinin raw material. The method employs a UV detector at 210 nm by using a C-18 column (Agilent Zobax ODS, 150 mm ~ 4.6 mm, 5 μm). The mobile phase consists of acetonitrile and water with different volume ratios and is controlled by a gradient program, in which 45% acetonitrile is kept for 10 min, followed by a linear 10 min gradient to 80% acetonitrile, a linear 5 min gradient to 45% acetonitrile, and maintaining 45% acetonitrile for 10min to recondition the column. Under above-mentioned conditions, the peaks of artemisinin, artemisitene and 9-epi-artemisinin can be separated each other completely. There are good linear relationships between peak area and concentration in the range of 4.11~28.76μg/mL for artemistene, and in the range of 9.42~65.93 μg/mL for 9-epi-artemisinim, the correlation coefficients (R) are all more than 0.998. The limits of detection ((3S/N)) are 2.668 ng and 75.34 ng, respectively. The recoveries by standard addition method are all in the range of 94%~ 108%.
出处 《湖南文理学院学报(自然科学版)》 CAS 2016年第1期24-28,34,共6页 Journal of Hunan University of Arts and Science(Science and Technology)
基金 湖南省教育厅资助科研项目(14A012) 湖南威嘉生物科技有限公司资助科研项目
关键词 青蒿素 脱氢青蒿素 9-表-青蒿素 高效液相色谱法 artemisinin artemisitene 9-epi-artemisinin high performance liquid chromatography(HPLC)
  • 相关文献

参考文献3

二级参考文献22

  • 1黄黎,刘菊福,刘林祥,李德凤,张毅,牛惠珍,宋红月,章春宜,刘晓宏,屠呦呦.中药青蒿的解热抗炎作用研究[J].中国中药杂志,1993,18(1):44-48. 被引量:26
  • 2PharmacologyLaboratoryinInstituteofTraditionalChineseMedicineofSichuanProvince(四川省中药研究所药理室).The pharmacology research of artemisinin(青蒿素的药理研究)[J].四川医学,1980,1:33-33.
  • 3Roth RJ,Acton N.Isolation of arteannuin acid from artemisia annua.Planta Med,1987,53:501.
  • 4TUYou-you(屠呦呦) NIMu-yun(妮慕云) ZHONGYu-rong(钟裕荣) etal.Study on the constituents of artemisia annual(中药青蒿素化学成分的研究)[J].药学学报,1981,16:366-366.
  • 5ChinaCooperativeResearchGroup(青蒿研究协作组).On Qinghaosu and its derivatives as antimalarials(抗疟药物青蒿素及其衍生物)[J].中草药,1982,2:45-45.
  • 6Filip CW,Van Nieuwerburgh,Sofie RF,et al.Quantitation of artemisinin and its biosynthetic precursors in Artemisia annua L.by high performance liquid chromatography-electrospray quadrupole time-of-flight tandem mass spectrometry.J Chromatogr A,2006,1118:180.
  • 7Ferreira JS,Janick J.Immunoquantitative analysis of artemisinin from Artemisia annua using polyclonalntibodies.Phytochemistry,1996,41(1):97.
  • 8Bharathi A,Yan-Hong W,Troy JS,et al.Comparison of LC-UV,LC-ELSD and LC-MS methods for the determination of sesquiterpenoids in various species of Artemisia.Chromatographia,2009,70:797.
  • 9Ma CF,Wang HH,Lu X,et al.Metabolic fingerprinting investigation of Artemisia annua L.in different stages of development by gas chromatography and gas chromatography-mass spectrometry.J Chromatogr A,2008,1186:412.
  • 10Chen J,Gu JK,Zhao R,et al.Simultaneous nonchiral determination of artemisinin and arteannuin B in Artemisia annua using circular dichroism detection.Chromatographia,2009,69:361.

共引文献53

同被引文献32

引证文献3

二级引证文献23

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部