卵泡刺激素和干细胞因子对大鼠非梗阻性无精子症模型生精功能的影响

Effect of FSH and SCF on spermatogenesis of non-obstructive azoospermia in rat models

目的探讨卵泡刺激素(FSH)、干细胞因子(SCF)对Wistar大鼠非梗阻性无精子症(NOA)动物模型生精功能的影响。方法使用白消安15mg/kg单次腹腔注射制作Wistar大鼠NOA动物模型,抽样检查模型制造成功后。随机抽取72只大鼠分为两组:治疗组给予大鼠后腿肌肉注射FSH 250mU/次和SCF 150ng/次,每3d重复注射1次,共注射19次;对照组予以等量的生理盐水行大鼠后腿肌肉注射。治疗组和对照组均于用药后19d、38d和57d摘取一侧睾丸制成石蜡病理切片,采用Johnsen法评价其生精功能;取一侧附睾计数附睾尾精子数,每组每次12只。结果治疗组用药19d、38d和57d睾丸组织切片Johnsen评分均显著高于对照组(P均<0.05);用药后19d和38d治疗组睾丸内曲细精管排列较对照组紧密、整齐;管腔内各级生精细胞数量亦多于对照组;用药后57d治疗组睾丸内曲细精管与正常睾丸基本一致,而同期对照组内有不少曲细精管局部呈现"空泡样"结构。用药后19d,两组附睾内均未见精子;用药38d时治疗组附睾内精子数[(77.55±55.18)个/ml]显著高于对照组[(41.05±29.20)个/ml](P<0.05);用药57d时治疗组附睾内精子数[(122.13±80.67)个/ml]亦显著高于对照组[(58.50±26.15)个/ml](P<0.05)。结论 FSH联合SCF肌注能够促进Wistar大鼠NOA模型生精功能的恢复。

Objective： To assessment the influence of FSH and stem cell factor（SCF）on the spermatogenic function in rat models of non-obstructive azoospermia.Methods： The 72 rat models of non-obstructive azoospermia were prepared by injecting busulfan at a dose of 15mg/kg into the abdominal cavity for each rat.The rat models were divided into two groups after successful establishment.The rats in treatment group were injected with 250 mU FSH and 150 ng SCF into hind leg muscle,every three days for 19 times.The rats in the control group were given equal amount of normal saline in the same way.The unilateral testis and epididymis of the rats were respectively obtained for morphological examination or sperm count on the 19 th,38th and 57 th day after administration.The spermatogenesis was evaluated by Johnsen score,and the sperm number in unilateral epididymis was accounted by CASA,twelve rats for each time.Results： The Johnsen scores of the rats in the treatment group were significantly higher than those in the control group on the 19 th,38th and 57 th day after administration（P〈0.05）.The arrangements of seminiferous tubule of the treatment groups were more tightness than the control groups,and the number of spermatogenic cells at all stage in the lumen in the treatment groups were more than the control groups on the 19 th or 38 th day after administration.The morphology of seminiferous tubules in the treatment group was similar as normal testis,while that in the control groups showed ＂ vacuole-like＂ structure on the 57 th day after administration.There was no sperm in the epididymis tube in both of treatment and control group on the 19 th day after administration.The sperm counts in the epididymis tube in treatment group on the 38 thday after administration [（77.55±55.18）/ml]were significantly higher than those in the control group [（41.05±29.20）/ml]（P〈0.05）,and it was similar on the 57 thday after administrating[（122.13±80.67）/ml vs.（58.50±26.15）/ml）（P〈0.05）.Conclusions： FSH and SCF by injecting muscle can improve the spermatogenesis recovery of Wistar rat with non-obstructive azoospermia.