多指标综合评分优选大孔树脂分离纯化三拗缓释片提取液工艺研究

Optimal separation and purification of extract in Sanao Sustained-release Tablets with macroporous resins by multi index comprehensive score

目的研究三拗缓释片提取液分离纯化工艺。方法以盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷和甘草酸的吸附率和解析率为考察指标,采用多指标综合评分法优选三拗缓释片提取液纯化工艺。结果 HPD 300大孔树脂对三拗缓释片中的4种有效成分具有较好的吸附效果,最佳工艺参数为每毫升树脂吸附1.67 g生药,树脂柱径高比1∶7,上样液质量浓度为生药0.6 g/m L,上样体积流量4.0 BV/h,2 BV去离子水洗涤,70%乙醇溶液洗脱,洗脱体积流量3.0 BV/h,洗脱量5 BV。结论 HPD 300大孔树脂分离纯化三拗缓释片提取液的方法可行。

Objective To explore the optimal separation and purification of extract in Sanao Sustained-release Tablets. Methods Taking the adsorption ratio and desorption ratio of ephedrine hydrochloride, pseudoephedrine hydrochloride, amygdalin, and glycyrrhizinate as evaluation indexes to optimize the purification process of Sanao Sustained-release Tablets by multi index comprehensive score. Results Macroporous resin HPD 300 had the best adsorption and desorption properties. In the course of adsorption, the optimum concentration of the sample liquid was 1.67 g crude drugs of per milliliter, the resin column size ratio was 1： 7, the concentration of sample solution was 0.6 g/mL crude drug, the sample flow rate was 4.0 BV/h. In the course of elution, 2 BV deionized water was used and the resin column chromatography was eluted with 5 BV of 70% Et OH by flow rate of 3 BV/h. Conclusion Macroporous resin HPD 300 is suitable to separate and purify the extract from Sanao Sustained-release Tablets.