药用植物组织培养中褐化现象的研究进展

Research progress on browning phenomenon in tissue culture of medicinal plants

药用植物外植体组织培养阶段,易受到各种因素的影响而产生褐化现象,这不仅影响药用植物外植体的鲜活度,而且还干扰了愈伤组织的诱导和再生,严重阻碍了药用有效成分的快速积累和组培技术的成功率。因此,研发快速、有效、安全、经济的褐化抑制方法显得尤为重要。对药用植物褐化现象、褐化机制、褐化影响因素及防褐有效措施进行了综述,以期为药用植物组织培养研究中抑褐措施提供科学参考,并对药用植物组织培养防褐技术进行展望。

Browning of explants may be easily induced by various factors during tissue culture of medicinal plants, which not only affects the vitality of explants, but also inhibits the callus induction and regeneration, blocking quick accumulation seriously of active components and survival rate of tissue culture. Therefore, it would be important to develop a fast, effective, safe, and economic browning restraining method. This paper reviewed the status, mechanism, and impact factors of the medicinal plants browning and effective anti-browning measures, expecting to provide the scientific references for development of anti-beowning measures. Meanwhile, we prospect anti-browning technologies in the tissue culture of medicinal plants.