黄皮茎枝化学成分研究

Chemical constituents from stems of Clausena lansium

目的研究黄皮属植物黄皮Clausena lansium茎枝的化学成分。方法采用大孔吸附树脂、硅胶柱色谱、反相柱色谱、凝胶柱色谱以及制备HPLC等方法进行分离纯化,运用紫外光谱、质谱和核磁共振谱等方法鉴定化合物的结构。结果从黄皮茎枝的95%乙醇提取物的正丁醇萃取部位分离得到15个化合物,分别鉴定为1,1′,1″,1′′′,1′′′′-三十碳内五酰胺（1）、4-羟基-2,6-二甲氧基苯酚-6′-O-紫丁香酰-β-D-吡喃葡萄糖苷（2）、4-羟基-2,6-二甲氧基苯酚-6′-O-香草酰-β-D-吡喃葡萄糖苷（3）、4-羟甲基-2-甲氧基苯酚-6′-O-紫丁香酰-β-D-吡喃葡萄糖苷（4）、4-羟基-2-甲氧基苯酚-6′-O-紫丁香酰-β-D-吡喃葡萄糖苷（5）、紫丁香苷（6）、松柏苷（7）、3,4,5-三甲氧基苯酚-O-β-D-吡喃葡萄糖苷（8）、tinotuberide（9）、（E）-异松柏苷（10）、苯乙基-O-β-D-吡喃葡萄糖苷（11）、araliopsine（12）、geibalansine（13）、全缘喹诺酮（14）、γ-花椒碱（15）。结论化合物1~14为首次从该种植物中分离得到,化合物1~11为首次从该属植物中分离得到,化合物1首次从天然产物中分离得到。

Objective To study the chemical constituents from the stems of Clausena lansium. Methods The chemical constituents were separated and purified by macroporous resin, silica gel, ODS column chromatography, Sephadex LH-20, and preparative HPLC. Their structures were determined by the analyses of ultraviolet spectrum, mass spectrum, and nuclear magnetic resonance spectroscopy. Results Fifteen compounds were isolated from the n-Bu OH fractions of 95% ethanol extract from the stems of C. lansium, and their structures were identified as 1,1′,1″,1′′′,1′′′′-tricontane lactam（1）, 4-hydroxy-2,6-dimethoxyphenyl 6′-O-syringoyl-β-Dglucopyranoside（2）, 4-hydroxy-2,6-dimethoxyphenyl 6′-O-vanilloyl-β-D-glucopyranoside（3）, 4-hydroxymethyl-2-methoxyphenyl-6′-O-syringoyl-β-D-glucopyranoside（4）, 4-hydroxy-2-methoxyphenyl-6-O-syringoyl-β-D-glucopyranoside（5）, syingin（6）, coniferin（7）, 3,4,5-trimethoxyphenyl-O-β-D-glucopyranoside（8）, tinotuberide（9）, trans-isoconiferin（10）, phenethyl-O-β-D-glucopuranoside（11）, araliopsine（12）, geibalansine（13）, integriquinolone（14）, and γ-fagarine（15）. Conclusion Compounds 1—14 are isolated from this plant for the first time, compounds 1—11 are isolated from the plants of genus Clausena L. for the first time, and compound 1 is a new natural product.